Create stunning time-lapse images in your lab

Designed specifically for the EVOS M5000 Imaging System and EVOS M7000 Imaging System, the EVOS Onstage Incubator is an environmental chamber that enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and non-physiological conditions.

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Application guide: Setting up hypoxic conditions with the EVOS onstage incubator. Download now

Superb flexibility, ease of use, and optical performance for live cell imaging

Environmental settings and image acquisition parameters are all seamlessly integrated into the EVOS M5000 and EVOS M7000 interfaces, creating a high-performance inverted imaging system with superb flexibility, ease of use and optical performance for demanding live cell time-lapse imaging experiments. Features include:

  • Fully integrated environmental chamber for live cell time-lapse imaging
  • Intuitively set environmental and image acquisition parameters
  • Easily maintain physiological or non-physiological conditions with precision control
  • Small footprint, robust build, and sleek design
  • Choice of a range of vessel holders

Time-lapse videos using the EVOS FL Auto 2 with Onstage Incubator

Cell division in HeLa cells

Cells: HeLa
Reagents: CellLight H2B GFP, CellLight Mito RFP
Time Lapse: 24 hours with images taken every 12 minutes

Imaged on EVOS FL Auto Imaging System using the Onstage Incubator

Disruption of microfilament organization in HeLa cells

Cells: HeLa treated with Latrunculin A
Reagents: CellLight Actin GFP, MitoTracker Red, NucBlue Live
Time Lapse: 14 hours with images taken every 5 minutes

Imaged on EVOS FL Auto Imaging System using the Onstage Incubator


Compatible VesselsWell plates, 35 mm dishes, T25 Flasks
Temperature RangeAmbient - 40oC
CO2 % Range0-20%
O2 % RangeAmbient - 0%
Humidity Range>80% RH @ 37oC
  • 25 x 19 x 3.7 cm (Environment chamber)
  • 37 x 16 x 20 cm (Control unit)
  • 1.5 kg (Environment chamber)
  • 10 kg (Control unit)

For Research Use Only. Not for use in diagnostic procedures.