When you order your EVOS M7000 system or M5000, you can choose from more than 20 available LED light cubes that are interchangeable between the two systems.
Choose light cubes whose spectra best match the dyes you want to use. Light cubes are easy to swap in and out and can be purchased at any time to meet new application requirements A wide range of dyes is supported.
Many customers choose some combination of DAPI, GFP, RFP or Texas Red, and Cy5 or Cy7. The table shows common dyes detected by these six popular light cubes.
|Light cube||Color||Excitation (nm)||Emission (nm)||Compatible dyes||Category number|
|DAPI||Blue||357/44||447/60||DAPI, Hoechst, NucBlue, Alexa Fluor 350, BFP||AMEP4950|
|GFP||Green||470/22||525/50||GFP, Alexa Fluor 488, Calcein, SYBR Green, FITC, Fluo-4, MitoTracker Green, pHrodo Green||AMEP4951|
|RFP||Orange||531/40||593/40||RFP, Alexa Fluor 546/555, PE, Cy3, MitoTracker Orange, Rhodamine Red, SYTOX Orange||AMEP4952|
|Texas Red||Red||585/29||628/32||Texas Red, Alexa Fluor 568/594, MitoTracker Red CMXRos, mCherry, pHrodo Red, Cy3.5, CellTracker Red CMTPX||AMEP4955|
|Cy5||Deep Red||628/40||685/40||Cy5, Alexa Fluor 647/660, DRAQ5||AMEP4956|
|Cy7||Near-IR||710/40||809/81||Cy7, IRDye® 800CW, DyLight 750||AMEP4667|
We now offer newly updated and optimized EVOS light cubes that feature improved opto-mechanical design and spectrally optimized optical components. The new design results in superior illumination uniformity across the field of view and maximizes signal-to-background ratios even with dim samples. The cubes are backward compatible with the legacy EVOS light cubes described above, and can also be used with the Browse Countess 3 FL Automated Cell Counter for expanded interchangeability.
¹ Applies to EVOS M7000 and M5000 only. With EVOS FLoid, standard blue, green, and red LED light sources are included. With EVOS XL Core, a standard white LED light source is included.
When you order your EVOS M7000 or M5000, you can choose among more than 30 high-performance objective lenses ranging from 1.25–100x. All EVOS objectives offer outstanding optical performance from visible light to near-infrared light. The extensive choice of objectives spans a full range of magnifications and optical specifications to satisfy your application requirements.
Microscopy objectives come in three major types, corresponding to their level of correction for chromatic aberrations:
Fluorite objectives are ideal for fluorescence and demanding transmitted-light applications. They provide excellent resolution, resulting in bright fluorescence signal and high-contrast imaging. Fluorite objectives help reduce optical aberrations. Color and focus have a higher level of correction than with other objectives.
Achromat objectives are ideal for general applications. Color and focus have standard correction compared to apochromat and fluorite objectives.
Apochromat objectives are ideal for the most demanding applications, especially capturing color images in white light. They provide the highest level of resolution, fluorescence brightness, contrast, and chromatic correction compared to achromat and fluorite objectives.
Within these types, objectives come in in two classes:
Long working distance (LWD) objectives are optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates with nominal wall thickness of 0.9–1.5 mm.
Coverslip-corrected (CC) objectives are optimized for imaging through #1.5 coverslips (approximately 0.17 mm thick). They have a higher magnification-to-numerical aperture (NA) ratio and provide higher resolution than LWD objectives.
Choose objectives with optics that match the type of imaging, type of sample, and vessel you will be using. (As with light cubes, you can always add additional objectives later.) Here are some general guidelines:
For a list of available objectives, see our objectives selection guide or download our Objectives Brochure. For a more detailed explanation of which objectives are best suited to which applications, including a handy decision tree that will help you choose, download our Selection Guide for EVOS Objectives.
²EVOS M7000 and M5000 only. With EVOS FLoid, a standard 20x plan fluorite objective is included. With EVOS XL Core, standard 4x, 10x, 20x, and 40x plan LWD phase, infinity objectives are included and a 2x (non-phase) objective can be ordered as an option.
Fluorescence imaging of live cells over time allows the study of dynamic processes and events that may unfold over hours or days. However, despite advances in both reagents and instrumentation, live-cell imaging remains challenging for two fundamental reasons: mammalian cells are highly sensitive to light, and they require stable environmental conditions.
Utmost care must be taken to protect cells from excessive light, and to maintain stable conditions of temperature, levels of atmospheric gases (O² , CO² , and N²), humidity, pH, and osmotic concentration (osmolarity) to ensure that the cells being observed remain healthy. Time-lapse imaging compounds these challenges because of the multiple light exposures and extended time out of the incubator.
Celleste Image Analysis Software (version 5.0) is a full-feature image analysis suite designed for a range of biological applications, from image adjustments and processing with manual and automatic measurements over multiple channels, to segmentation and classification tools that help you transform images into quantitative data in a streamlined and customizable workflow. Optional modules for 2D and 3D deconvolution, 3D visualization, and 3D analysis allow you to customize the capabilities according to your needs.
EVOS vessel holders and stage plates fit a wide range of vessels, flasks, plates, dishes, and slides. Custom configurations are also available upon request.