The central feature shared by all EVOS cell imaging systems is their remarkable image quality. In just a few clicks, capture clear, bright, publication-quality images and videos that help you tell the story of your data.
All EVOS imaging systems feature bright LED light sources that provide more than 50,000 hours of brilliant (yet adjustable) illumination that (unlike mercury arc lamps) remains constant with minimal degradation over time. Cost-efficient and environmentally friendly, EVOS LED light cubes are designed for precise control, minimal maintenance, and exceptional reliability.
For the M7000 and M5000, a choice of compact, easily interchangeable LED light cubes can be matched to the spectra of specific dyes or fluorescent proteins in your application. Each cube incorporates excitation filter, dichroic beam-splitter, and emission filter. Filters are hard-coated for maximum transmission efficiency.
EVOS now offers newly updated and optimized light cubes that feature improved opto-mechanical design, spectrally optimized optical components, and best-in-class spectral fidelity and stitching performance. For more information, see the Accessories.
Mercury arc lamps can lose 50% of their intensity in the first 100 hours of operation! In addition, images acquired in different sessions cannot be quantitatively compared using without complicated calibrations. Because EVOS LED light sources produce constant light intensity, users can rely on consistent illumination and can compare results quantitatively from images acquired on different days.
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb (and 1,500 hours for a metal halide bulb). That means significant savings in the overall upkeep of your instrument.
Hard-coated filter sets are more expensive but have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS system’s hard-coated filter sets, your light cubes cost less over time—and provide brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.
Depending on the model, EVOS cell imaging systems include either a high-sensitivity monochrome camera, ideal for fluorescence imaging; a high-sensitivity color camera, ideal for colorimetric imaging; or both monochrome and color cameras for maximum flexibility.
A choice of more than 30 high-performance objective lenses ranging from 1.25–100x allows you to match the optics to the type of imaging, type of sample, and vessel. Image acquisition is speedy, and with automated multiwell screening the M7000 can scan a 96-well plate in 3 fluorescence channels in less than 5 minutes. EVOS cell imaging systems make it easy to capture publication-quality images in seconds.
EVOS microscopes are designed with scientists’ workspace and workflow needs in mind. Components and controls are conveniently integrated into a single, lightweight system that fits on a benchtop and can easily be moved to classrooms, teaching labs, and conference rooms. The size and portablility of EVOS microscopes let you capture and view images when and where needed.
With all EVOS imaging systems, a large, bright, high-definition monitor lets you view your cells outside the confines of a darkroom and facilitates safe collaboration in nearly any space. There’s no need to remove safety goggles to look through an ocular eyepiece. This minimizes difficult inter-user decontamination and facilitates both collaboration and safety.
The EVOS M5000, FLoid, and XL Core are designed for use in a biosafety cabinet or hood while wearing gowns, gloves, and goggles or face shields if necessary. There’s minimal risk of contamination when you passage, split, transfect, transduce, or otherwise manipulate your cells.
Further, with the advanced EVOS imaging systems, software controls most or all microscope operations. You can adjust focus, zoom, pan, and (with the EVOS M7000) even control stage movement remotely through software, without touching the instrument, minimizing the possibility of infection or cross-contamination through physical contact. In addition, with the M7000, you can run automated routines such as multiwell plate scanning and successive scans of a designated area. The only required interaction with the instrument is to mount the slide, plate, or other vessel.
All EVOS microscopes come with onboard software that allows you to view, capture, and process images quickly and easily. You can:
On the M7000 and M5000, the onboard software offers pinpoint operational control and powerful image processing tools such as cell counting, confluence, Z-stacking, image tiling and stitching, time-lapse movies, and RGB synthesis. These tools operate intuitively with minimal training, aided by onboard SmartStart guides. Autofocus and other automated tools save time and effort. The M7000 adds advanced automation features and complete programmatic control, allowing imaging through software alone, without ever touching the instrument. Download the most recent EVOS M7000 and M5000 software updates.
For advanced image processing and analysis, the Celleste Image Analysis Software option offers powerful additional tools to segment, count, size, classify and analyze complex images, including machine learning algorithms for advanced image analysis and quantitation. Optional modules for deconvolution, 3D visualization, and 3D analysis allow you to customize the capabilities according to your needs.
Images from all EVOS instruments can be sorted and analyzed with the EVOS Image Analysis app in Connect, Thermo Fisher’s secure, cloud-based platform for data storage, scientific analysis apps, and peer collaboration tools. Once uploaded, you and your colleagues can collaboratively access your gallery and retrieve, analyze, and manipulate your images from any web browser around the world. You can also run other Connect applications and monitor the status and configuration of your connected instruments.
In the Image Analysis app, you can zoom and pan images, adjust the brightness and contrast of each channel, and apply pseudocolors. You can also overlay a region and display its dimensions. When analyzing an image, once you identify your target cells vs background, the app can automatically count the cells in a given area and quantify their area, fluorescence intensity, and circularity.
For Research Use Only. Not for use in diagnostic procedures.