Laser-based fluorescence as well as brightfield illumination and confocal capabilities

Catalog numbers: CX7A1110LZR, CX7B1112LZR, A37013, A37014

 

The Thermo Scientific CellInsight CX7 LZR platform seamlessly integrates advanced screening technologies to deliver superior performance for lightning-fast discovery across the life sciences. Designed to meet the challenging needs of emerging life science models, the CX7 LZR incorporates both laser-based fluorescence illumination and integrated Nipkow spinning-disc confocal technology for publication quality imaging of a broad range of samples from simple monolayers to thick spheroid samples.  

 

Dual mode software- and laser-based autofocusing methods contribute to the platform’s diverse capabilities to investigate simple to complex models.  On-the-fly phenotyping enables parallel image capture and analysis for multiplexed cytometry measurements in real time.  The Thermo Scientific Amira software option enables 3-dimensional analysis of the most complex research models from 3D morphological tracing in neurons to co-localizations of immune and cancer cells within tumors to enable next-generation translational research.  


Features

In addition to the common features of all high-content screening platforms, the CellInsight CX7 LZR platform offers:

Laser-based illumination 

Seven independent lasers aligned with multiplexable fluorescent cellular labeling for the best performance in speed and image quality.

Laser illumination provides flatter and brighter images with low background.

 

Autofocus

Dual Mode Autofocusing Software and Laser Autofocus modes accommodate the widest variety of sample types.

 

Extremely bright illumination

Penetrate thick samples during widefield or confocal 3D imaging.

Laser light engine

Illuminates the field evenly resulting in the best image quality appropriate for quantitation.

Up to 6X increase in 3D confocal screening times compared to LED illumination.

 

Short exposure times and laser autofocus capabilities

Speeds up the acquisition of images. 

 

Near-IR (785 nm) laser excitation

Expand your multiplexing capabilities with near-IR (785 nm) laser excitation.


Specifications

Category Attribute Description
Optics Camera

Photometrics high-resolution fluorescent camera:

  • High-resolution, thermoelectrically cooled to 0°C
  • FOV 2208 x 2208-pixel array
  • 4.54 μm pixel size
  • 75% peak quantum efficiency (QE)
  • Multiple pixel binning options for flexible analysis options
  Light source High-output, laser-based illumination. Configured for imaging in the UV range through near-IR range with the provided filter sets.
  Objectives

Standard (Olympus™ objectives):

  • 10x (0.30 NA) Plan NeoFluar™ system
  • 20x (0.4 NA) Achroplan™ system
  • 20x (0.7 NA) Olympus objectives
  • 40x (0.6 NA) Olympus objectives

Optional (Olympus objectives):

  • 2x (0.08 NA)
  •  4x (0.16 NA)
  • 40x (0.95 NA)
  • 60x (0.90 NA)
  Image compatibility JPG, BMP, GIF, PNG, TIF, C01, DIB
Physical characteristics Dimensions 20 in. x 32 in. x 18 in. (50.8 cm x 81.3 cm x 45.7 cm)
  Weight 68 kg (150 lb)
System Data management Compatible with Thermo Scientific Store Image and Database Management Software
  Software Thermo Scientific HCS Studio 4.0 Cell Analysis Software including EurekaScan Finder double-pass functionality
  PC

Windows 10 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel® Xeon® Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.

Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse

  Wattage 300 W

Videos and demos

Introducing the Thermo Scientific CellInsight CX7 LZR High-Content Analysis Platform


Sample data

Immunofluorescence image of human neurons

Confocal image obtained with CellInsight CX7 LZR instrument. Neurons were fluorescently stained with both DAPI and MAP2 (560 nM) to counterstain the nucleus and cell body/neurites, respectively. (60X objective (0.9NA); Nipkow spinning disc at 40 micron pinhole setting)

Multicolor immunofluorescent imaging with confocal option from CellInsight CX7 LZR instrument.

Immunofluorescence image of HeLa spheroid

HeLa spheroids labeled with Ki67 antibody followed by Invitrogen Alexa Fluor 647 and Alexa Fluor 488 phalloidin and Hoechst 34580 stains for nuclei. Imaged on the CellInsight CX7 LZR instrument using a 10x objective and 70 micron pinhole setting.

Multicolor fluorescent image of spheroid imaged using CellInsight CX7 LZR and Invitrogen antibodies and dyes.

LED illumination

image23

Confocal scan

image22

3D confocal scan of spheroids is faster than LED illumination

 

3D, 4-color stacks were acquired on A549 spheroids using CellInsight Cx7 LZR with a 10x/0.4 NA objective. 70 µm confocal was used for all 4 colors (20 slices, 7 µm steps). Illumination was via 405, 488, 651, and 647 lasers. The scan improvement speed when using the CellInsight CX7 LZR  platform over the LED instrument was 5.8x for a 1-well scan and 6.1x for a 39-well scan.

Imaging proliferating cells in spheroids using CX7 LZR platform

Imaging and analyzing proliferating cells in spheroids using CX7 LZR HCS instrument.

In hydroxyurea treated spheroids, actively proliferating s-phase cells disappear (EdU negative)

Imaging and detecting spheroid size by segmenting and quantitating EdU and CX7 LZR platform

Segmentation and quantitation of EdU and spheroid size using CellInsight CX7 LZR system.

Spheroid segmented as whole object and EdU positive cells segmented in the spheroid for quantitation

Reagent selection guide

The Thermo Scientific CellInsight CX7 LZR high-content instrument allows you to take advantage of the entire fluorescent spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions.

Fixed cell labeling

Blue Green/Yellow Orange Red Deep Red Near IR
Alexa Fluor
405

Alexa Fluor
488
Alexa Fluor
532
Alexa Fluor
555
Alexa Fluor
594
Alexa Fluor
647
Alexa Fluor
750
Alexa Fluor
790
  Goat anti-chicken IgY   Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY    
Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG
Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG
  Goat anti-rat IgG   Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG    
  Goat anti-guinea pig IgG   Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG    
  Goat anti-human IgG   Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG    
  Donkey anti-goat IgG   Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG   Donkey anti-goat IgG
  Donkey anti-mouse IgG   Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG   Donkey anti-mouse IgG
              Donkey anti-rabbit IgG
  Donkey anti-rat IgG     Donkey anti-rat IgG      
  Rabbit anti-goat IgG   Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG   Rabbit anti-goat IgG
  Rabbit anti-mouse IgG   Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG   Rabbit anti-mouse IgG
  Rabbit anti-rat IgG     Rabbit anti-rat IgG      
  Chicken anti-goat IgG     Chicken anti-goat IgG Chicken anti-goat IgG    
  Chicken anti-mouse IgG     Chicken anti-mouse IgG Chicken anti-mouse IgG    
  Chicken anti-rabbit IgG     Chicken anti-rabbit IgG Chicken anti-rabbit IgG    
  Chicken anti-rat IgG     Chicken anti-rat IgG Chicken anti-rat IgG    

Live/fixable cell labeling

Violet Blue Green/Yellow Orange Red Deep Red
BFP CFP GFP
emGFP
YFP RFP
mOrange
DsRed
mCherry
 

For Research Use Only. Not for use in diagnostic procedures.