High-Content Screening (HCS)

High-content screening (HCS) or high-content analysis (HCA) describes a set of analytical methods using automated microscopy, multi-parameter image processing, and visualization tools to extract quantitative data from cell populations. HCS typically employs fluorescence imaging of samples in a high-throughput format and reports quantitatively on parameters such as spatial distribution of targets and individual cell and organelle morphology. Thermo Scientific CellInsight CX5, CellInsight CX7 and CX7 LZR, and ArrayScan high-content analysis products have directly impacted every segment of biological research into cellular and systems biology and drug discovery.

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Invitrogen HCA Onstage Incubator

Invitrogen HCA Onstage Incubator

The Invitrogen High-Content Analysis (HCA) Onstage Incubator allows you to equip your Thermo Scientific CellInsight instrument with live-cell imaging capabilities, including the option to upgrade your existing CellInsight platform.*

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* Please contact your Thermo Fisher Scientific representative for details. Instruments that qualify for upgrade include: Thermo Scientific CellInsight CX5 instruments (2015–present), CellInsight NXT instruments (2012–2015), and CellInsight CX7 instruments (2015–present). 

High-content analysis reagents

BPAE cells imaged using the CellInsight™ CX5

BPAE cells imaged using the Thermo Scientific CellInsight CX5 platform and stained with DAPI (blue), Invitrogen Alexa Fluor 568 phalloidin (red), goat anti–mouse IgG (H+L) secondary antibody, and Alexa Fluor 488 conjugate (green).

A549 cells imaged using the CellInsight CX7 platform

A549 cell image acquired using the CellInsight CX7 platform and stained with Invitrogen HCS CellMask Blue (blue), Alexa Fluor 488 Phalloidin (green), and Alexa Fluor 750 goat anti–mouse IgG secondary antibody (pink).

HT1080 cell image acquired using an ArrayScan™ reader

HT1080 cell image acquired using an ArrayScan system and stained with DAPI (blue), Invitrogen Alexa Fluor 555 phalloidin (red), and anti-tubulin and Alexa Fluor 647 goat anti–mouse IgG antibodies (green).

HCS reagent selection guides

Drawing on decades of experience in fluorescence imaging, Invitrogen HCS products are developed using Thermo Scientific high-content platforms with special considerations for the high-throughput workflow and automated imaging:

  • Cell and nuclear masks for automated demarcation
  • Robust functional probes for cell health interrogation
  • Flexible assay workflow for automated processing
  • Broad fluorophore choice for easy multiplexing
  • Validated on multiple imaging platforms

Select from the reagents optimized for use with your HCS platform:

Other cell structure and segmentation tools

Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Invitrogen Molecular Probes cell structure labels are widely used in microscope imaging and HCS.

See reagents for cell structure

Other cell health and cytotoxicity assays

We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.

Browse cell health and cytotoxicity assays by application: