Optimized, automated assays for HCS
Measuring the synthesis of new DNA is the most precise way to assay cell proliferation in individual cells, cell populations, or tissues. This automated assay measures new DNA synthesis based on incorporation of the nucleoside analog EdU into DNA. A copper-catalyzed “click” reaction adds a fluorescent Invitrogen Alexa Fluor dye conjugate to the EdU and enables detection with a range of wavelength choices for easy multiplexing. With a nuclear stain for the detection of all cells and an Alexa Fluor dye to label newly synthesized DNA, proliferation can be measured in a two-channel assay. All the remaining channels are available to measure other cellular parameters.
- 10x or 20x magnification
Automatically measured properties
- Total cells
- Replicating cells
Cell proliferation assay images
A549 cell proliferation assay. A549 cells were treated with increasing doses of paclitaxel. Cells were stained with Invitrogen Click-iT Plus EdU Alexa Fluor 647 Imaging Kit (red) and Invitrogen Hoechst 33342 stain (blue). Cells were imaged using the Thermo Scientific CellInsight CX7 High-Content Analysis Platform, and total cells and replicating cells were measured using Thermo Scientific HCS Studio Software.
Sample experimental data
Dose-response plots of U2OS cells treated with increasing concentrations of proliferation inhibitors and stained using Invitrogen Molecular Probes Click-iT Plus EdU labeled with Invitrogen Molecular Probes Alexa Fluor 647 dye.
For Research Use Only. Not for use in diagnostic procedures.