photo of the CellInsight™ CX5 High-Content Screening (HCS) Platform

The Thermo Scientific™ CellInsight™ CX5 High-Content Screening (HCS) Platform is a compact, affordable screening system for researchers looking to increase scale and capacity in everyday use.

Compact and affordable high-content screening platform

Increase your screening throughput with CellInsight CX5 Platform

With the compact and powerful CellInsight CX5 High-Content Screening Platform you can read and analyze a 96-well plate in under four minutes. Designed for researchers looking to increase their screening throughput, the platform offers common excitation wavelengths for fluorescence and white light illumination for colorimetric samples. The system is ready for full automation with 96-, 384-, or 1,536-well plates, or you can analyze your tissue sections one slide at a time to work up your next assay.

CellInsight CX5 system highlights

Illumination 5-channel solid-state LED light engine
Camera Photometrics X1 camera 
Widefield 5-channel fluorescent imaging
Brightfield White light brightfield imaging
Objectives Single-position objective (range from 2x–20x, low and high NA)
Focus Software autofocus
Automation Configured for fully automated plate handling and scanning
Software HCS Studio software for integrated data collection and analysis
BPAE cells imaged using the CellInsight CX5 platform

BPAE cells imaged using the CellInsight CX5 platform and stained with DAPI (blue), Alexa Fluor 568 phalloidin (red), and goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (green).

HeLa cells imaged using the CellInsight CX5 platform

HeLa cells imaged using the CellInsight CX5 platform and stained with Hoechst™ 33342 (blue) and Alexa Fluor 680 phalloidin (yellow).

White light brightfield image of a fixed tissue slice imaged with the CellInsight CX5 platform.

White light brightfield image of a fixed tissue slice stained with Hematoxylin and Eosin imaged with the CellInsight CX5 platform.

Reagents optimized for high-content screening

Drawing on decades of experience in fluorescence imaging, our HCS products are developed using Thermo Scientific High-Content Platforms with special considerations for the high-throughput workflow and automated imaging:

  • Cell and nuclear masks for automated demarcation
  • Robust functional probes for cell health interrogation
  • Flexible assay workflow for automated processing
  • Broad fluorophore choice for easy multiplexing
  • Validated on multiple imaging platforms

Select reagents for use with the CellInsight CX5

Other cell structure and segmentation tools

Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Molecular Probes cell structure labels are widely used in microscope imaging and HCS.

See other reagents for cell structure

Other cell health and cytotoxicity assays

We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.

Browse cell health and cytotoxicity assays by application:

Microplates and slides

Compatible with SBS standard microplates (6–1,536 well) and slides


Orbitor RS Microplate Mover

Increase throughput, storage capacity and operational flexibility with the Thermo Scientific Orbitor RS Microplate Mover—industrial-sized performance within a compact, benchtop mover.

The Orbitor RS is a high-speed microplate mover offering proven, reliable performance with totally flexible plate handling. Extensive vertical reach allows multiple stacked or high density instruments to be loaded in a small footprint, and a bi-directional telescoping arm provides superior reach, improved user safety and unlimited base rotations within a 360° workspace.

Camera Photometrics X1 CCD:
  • High-resolution, thermoelectrically cooled to 0°C
  • 14-bit dynamic range
  • FOV 2208 x 2208 pixel array
  • 4.54 micron pixel size
  • 75% peak Quantum Efficiency (QE)
Data management Compatible with Thermo Scientific Store Image and Database Management Software
Dimensions Height: 37.2 cm (14.6 in)
Width: 41.4 cm (16.3 in)
Depth: 59.4 cm (23.4 in)
Image compatibility JPG, BMP, GIF, PNG, TIF, C01, DIB
Light source LED, solid-state five-color light engine used with provided filter sets offers the following excitation/emission capabilities (Ex/Em, in nm):
  • 386/440 (e.g., Alexa Fluor 350 dye)
  • 485/521 (e.g., Alexa Fluor 488 dye)
  • 560/607 (e.g., Alexa Fluor 594 dye)
  • 650/694 (e.g., Alexa Fluor 647 dye)
  • 740/810 (e.g., Alexa Fluor 750 dye)
Objectives Standard (Olympus objectives):
  • 10X (0.30NA)
  • 20X (0.4NA)
Optional (Olympus objectives):
  • 2x (0.08NA)
  • 4x (0.16NA)
PC Windows 7 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel™ Xeon™ Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.
Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse.
Software Thermo Scientific HCS Studio 3.0 Cell Analysis Software
Wattage 300 W
Weight 45 kg (99.2 lb)

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