With the CellInsight™ CX5 high-content instrument you can take advantage of the entire fluorescent spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. Use the tables below to select reagents for each channel; they are divided into application areas, including products for segmentation, cell structure, and more.

  • Select the Fixed cells or Live/fixable cells tab to see a reagent selection that matches your detection protocol.
  • Click on individual product links (within colored cells) to see product details including spectral profiles, images, sample data sets, citations, and cell-based protocols.
  • Click on an application area to see selection guides for additional fluorescence-based tools and assays for imaging.
Blue
Green
Orange/Red
Deep Red
Near IR

Antibody labels for secondary detection

Alexa Fluor® 350 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750
  Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY  
Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG
Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG
Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG  
  Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG  
  Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG  
Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG  
  Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG  
  Donkey anti-rat IgG   Donkey anti-rat IgG    
  Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG  
Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG  
  Rabbit anti-rat IgG   Rabbit anti-rat IgG    
  Chicken anti-goat IgG   Chicken anti-goat IgG Chicken anti-goat IgG  
  Chicken anti-mouse IgG   Chicken anti-mouse IgG Chicken anti-mouse IgG  
  Chicken anti-rabbit IgG   Chicken anti-rabbit IgG Chicken anti-rabbit IgG  
  Chicken anti-rat IgG   Chicken anti-rat IgG Chicken anti-rat IgG  

Cell segmentation

Whole cell segmentation
HCS CellMask™ Blue HCS CellMask™ Green HCS CellMask™ Orange   HCS CellMask™ Deep Red  
Nuclear segmentation
HCS NuclearMask™ Blue SYTO® 9 Green fluorescent nucleic acid stain SYTO® 82 Orange fluorescent nucleic acid stain HCS NuclearMask™ Red HCS NuclearMask™ Deep Red  
DAPI SYTOX® Green nucleic acid stain   HCS NuclearMask™ Red TO-PRO®-3 stain  

Cell structure

Cytoskeleton—actin
Alexa Fluor® 350 phalloidin Alexa Fluor® 488 phalloidin Alexa Fluor® 555 phalloidin Alexa Fluor® 594 phalloidin Alexa Fluor® 647 phalloidin  
Plasma membrane
Wheat germ agglutinin, Alexa Fluor® 350 conjugate Wheat germ agglutinin, Alexa Fluor® 488 conjugate Wheat germ agglutinin, Alexa Fluor® 555 conjugate Wheat germ agglutinin, Alexa Fluor® 594 conjugate Wheat germ agglutinin, Alexa Fluor® 647 conjugate  
Blue
Green
Orange/Red
Deep Red

Cell segmentation

Plasma membrane segmentation
  CellMask™ Green plasma membrane stain CellMask™ Orange plasma membrane stain   CellMask™ Deep Red plasma membrane stain
Nuclear segmentation
HCS NuclearMask™ Blue SYTO® 9 Green fluorescent nucleic acid stain SYTO® 82 Orange fluorescent nucleic acid stain HCS NuclearMask™ Red HCS NuclearMask™ Deep Red

Cell tracking & tracing

CellTracker™ Blue CellTracker™ Green CellTracker™ Orange CellTracker™ Red CellTracker™ Deep Red

Cell structure

Cytoskeleton—tubulin
  Tubulin Tracker™ Green      
Endoplasmic reticulum
  ER-Tracker™ Green (BODIPY® FL Glibenclamide)   ER-Tracker™ Red (BODIPY® TR Glibenclamide)  
Lysosomes
      LysoTracker® Red DND-99  
Mitochondria
    MitoTracker® Orange CMTMRos MitoTracker® Red CM-H2XRos MitoTracker® Deep Red FM

Cell function probes

Cell viability
  Image-iT® DEAD™ Green viability stain   Propidium iodide  
Cell proliferation
  Click-iT® EdU Alexa Fluor® 488 HCS Assay Click-iT® EdU Alexa Fluor® 555 HCS Assay Click-iT® EdU Alexa Fluor® 594 HCS Assay Click-iT® EdU Alexa Fluor® 647 HCS Assay
Apoptosis—caspase activity
  CellEvent® Caspase-3/7 Green Detection Reagent      
Apoptosis—DNA fragmentation TUNEL
  Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay   Click-iT® TUNEL Alexa Fluor® 594 Imaging Assay Click-iT® TUNEL Alexa Fluor® 647 Imaging Assay
Oxidative stress—general
  CellROX® Green reagent     CellROX® Deep Red reagent
Endocytosis—lysosome-directed
  pHrodo™ Green Dextran, 10,000 MW for endocytosis pHrodo™ Red Dextran, 10,000 MW for endocytosis    
  Dextran, Alexa Fluor® 488, 10,000 MW Dextran, Alexa Fluor® 555, 10,000 MW Dextran, Alexa Fluor® 594, 10,000 MW Dextran, Alexa Fluor® 647, 10,000 MW
Endocytosis—receptor-mediated
  Transferrin from human serum, Alexa Fluor® 488 conjugate     Transferrin from human serum, Alexa Fluor® 647 conjugate
Cell cycle
  Premo™ FUCCI Cell Cycle Sensor Premo™ FUCCI Cell Cycle Sensor    
Autophagy
  Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B    

New protein synthesis

Click-iT® HPG
  Alexa Fluor® 488 Azide Alexa Fluor® 555 Azide Alexa Fluor® 594 Azide Alexa Fluor® 647 Azide
Click-iT® AHA
  Alexa Fluor® 488 Alkyne Alexa Fluor® 555 Alkyne Alexa Fluor® 594 Alkyne Alexa Fluor® 647 Alkyne
Click-iT® OPP
  Alexa Fluor® 488 Picolyl Azide Toolkit Alexa Fluor® 555 Picolyl Azide Toolkit   Alexa Fluor® 647 Picolyl Azide Toolkit