The Tali® Image-Based Cytometer is a benchtop assay platform giving you quick, quantitative analysis of GFP/RFP expression, cell cycle, apoptosis, cell viability, and much more right from your benchtop. Find the latest, up-to-date software and firmware here and keep your Tali® Image Cytometer running at maximum performance.

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Software Update Log

Version No. Released  
2.1 (latest)10/30/2012 Download now [.zip, 4.3 MB]

NOTE: Please contact Life Technologies Technical Support if you are updating from software version


Major Update—Tali® Software Update v2.1

What’s new in version 2.1:

Cell Cycle Analysis
The Tali® Image-Based Cytometer can perform cell cycle analysis using propidium iodide stained cells.  Additionally, the resulting cell cycle data can be downloaded in Excel-compatible or FCS format.  The FCS formatted data can be transferred to any flow cytometry–modeling software.

Fine tuning of fluorescent thresholds
Now you can use an on-screen keypad to adjust the fluorescent thresholds. This new feature allows for increased control and easier experiment-to-experiment comparison.

The X-axis can be adjusted
You can easily adjust the x-axis and expand or zoom into the region of interest. This helps to separate and identify low fluorescent expressing cells.

Auto-alignment capability
Instead of a manual alignment of the cameras, you insert the Tali® fluorescent alignment beads and run the auto-alignment program. This results in very consistent data by reducing user-to-user variability.

Tali® Software Update Info and Instructions

Attention—Before You Install

As a precaution when installing any update, save your vital data from the Tali® Image-Based Cytometer:

  1. Insert a USB drive into the Tali® Image-Based Cytometer
  2. Press the ‘Data’ tab at the top of the screen
  3. Select the files you wish to save to the USB drive
  4. Select Export format; choose either Data tables (.csv), Images (.jpg), Report (.pdf) or All formats
  5. When the file(s) transfer is complete, remove the USB drive and transfer files to your computer


Step 1—Download and save to a clean USB drive:

Download the software and save it to a clean USB drive.

a) Press the download button and save the Tali® Software zip file to your computer.

b) Obtain a USB Drive and remove all files or programs from this drive. Existing files can interfere with the software installation process thus it is important to remove all files or programs from the USB drive before continuing.

c) Using the clean USB drive, place the three programs and the image file from the zip file onto the USB drive.

d) After the transfer, review the file and program configuration of the USB Drive.  The configuration should be the following (see below).


Step 2—Transfer files to your Tali® Image Cytometer

Download the software and save it to your Tali® Image-Based Cytometer USB drive.

a) Insert the USB drive into the USB port located on the front of the Tali® Instrument.

b) Press the ‘Settings’ tab (highlighted with the red box below).


c) Press the 'Update firmware' button (highlighted with the red box below).


d) Wait until the Tali® instrument recognizes the USB drive and the green light is visible on the 'Update' button (see below).


e) Press the 'Update' button.

f) When the Update is complete the instrument will need to be restarted.  Press the ‘OK’ button (see below).


g) After the instrument performs the restart, press the ‘Settings’ tab and check that the software has been updated to the latest version (see below).



If you experience system instability when exporting multiple files, all data are preserved.  Turn the instrument off and then on to reinitialize the system.