Advancing meaningful discoveries

Invitrogen™ antibody conjugates for flow cytometry offer a diverse array of highly specific primary antibodies against intracellular and cell surface markers studied in both basic and clinical research settings. These extensive catalog of RUO and ASR primary antibodies are fully validated for flow cytometry and include multiple conjugates across a broad range of specificities to further assist and enable your research.

The new addition to this family are the Invitrogen™ eBioscience™ Super Bright polymer dyes. They join the Alexa Fluor™ dye and eFluor™ dye antibody conjugate portfolios to help answer complex cell biology questions—in less time and with less sample than other approaches. Search for your primary antibody conjugate and purchase with confidence with our antibody performance guarantee.

Find your flow cytometry antibody conjugate

Step 1. Search for the marker of interest (e.g., “CD4” or “CD45”).
Step 2. Narrow results by conjugate, target species, and other criteria using side filters.

Fluorochrome overview

The availability of flow cytometers equipped with lasers options beyond the standard 488 nm (blue) and 633 nm (red) lasers continues to grow as researchers strive to discover more about their research area from every sample analyzed. Newer lasers, namely violet, green, and yellow lasers are playing increasingly important roles in allowing researchers to increase the number of parameters investigated in a single sample to answer more complex biological questions. Information about fluorophore options for primary antibody conjugates for flow cytometry are available for each laser type.

Invitrogen™ fluorescence technologies have a long-standing history dating back to the development of the Alexa Fluor™ labeling technology in the 1970s. Today, the trusted Alexa Fluor™ and eFluor™ organic dyes, the classic fluorescent dyes, and the established tandem dyes are joined by our newest line of bright fluorochromes based on a polymer dye and its tandems that are excited by the violet laser, the Invitrogen™ eBioscience™ Super Bright dyes.

Super Bright polymer dyes

Super Bright polymer dyes are violet laser-excitable fluorophores that are designed to enable you to increase the number of markers off the violet channel for increased capabilities in multi-parametric flow cytometry analysis. These dyes typically require reduced compensation due to their tight spectral emissions, and exhibit increased detection of low-abundance antigens due to their super-bright signal. All of these properties combined are aimed at helping you detect more in a single sample and answer complex cell biology questions—in less time and with less sample.

The Super Bright polymer dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, and UltraComp eBeads™ microspheres. These features, combined with our broad portfolio of biological content, easily enable antibody conjugate selection for optimized flow cytometry multicolor antibody panel design and allow you to expand the utility of your violet laser.

Super Bright dye intensity

Figure 1. Fluorescence intensity comparison. (A) Human peripheral blood cells were stained with Anti-CD19 (clone HIB19) conjugated to either Super Bright 436 (purple histogram), eFluor 450 (blue histogram), or Brilliant Violet™ 421 (orange histogram) using the manufacturer’s recommended volume per test. (B) Anti-human peripheral blood cells were stained with Anti-CD27 (clone O323) conjugated to either Super Bright 436 (purple histogram) or Brilliant Violet 421 (blue histogram).

Super bright polymer dye emission spectra

Figure 2. Emisison spectra of Super Bright polymer dyes.

Classical fluorescent dyes, tandem dyes and proteins

A wide variety of fluorescent dye—conjugated primary antibodies is available that span the near-UV, visible, and near-IR spectrum, including antibodies conjugated to smaller organic dyes such as the Invitrogen™ Alexa Fluor™ dyes and the Invitrogen™ eBioscience™ eFluor™ dyes. Proteins which fluoresce naturally, and exhibit exceptional brightness or desirable excitation and emission spectral qualities, such as PE and APC, play a critical role in multiplex flow cytometry panels. Additionally, with over 10 different tandem dye combinations, (a tandem fluorochrome being the combination of two fluorescent molecules, typically a large protein-based dye (the donor) and a smaller organic dye (the acceptor), that are covalently linked), the classic fluorescent dyes, tandems and proteins provide a broad range of fluorochrome options that continue to be critical as advances in flow cytometry instrumentation technology allow more and more fluorescent colors to be used.

Data image of 13-parameter immunophenotyping
click to enlarge

Figure 3. Multiplex analysis using classic fluorescent and tandem dyes. Gating stragey for 13-parameter immunophenotyping of human lysed whole blood with the Invitrogen™ Attune™ NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells. For more details on this panel, download the application note.

 

Antibody labeling

We offer a number of labeling kits for the direct attachment of Alexa Fluor dyes, Qdot nanocrystals, R-phycoerythrin (R-PE), or even biotin to IgG antibody at amounts of less than 10 μg up to 1 mg. Directly labeled antibodies allow you to use more than one same species antibody in a single staining experiment. You can use traditional labeling chemistries optimized for your application or site-specific labeling using click-chemistry technology. Find out more about antibody and protein labeling kits including products that use our patented Zenon™ labeling technology or the SiteClick™ labeling technology.

Custom fluorescent antibody labeling

Our eBioscience custom services group has the expertise you need for antibody conjugation, antibody cocktails, immunoassay development, bulk reagents, testing services and more.

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