Brilliant Ultra Violet™ dyes increase options

Which buffer should I use with Brilliant dyes?

Either the Super Bright Staining Buffer or Brilliant Staining Buffer will work to help resolve polymer dye-to-dye interactions.

Brilliant Ultra Violet™ Dyes

Brilliant Ultra Violet™ dyes (BUVs) are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. BUVs are dyes used on flow cytometers equipped with 355 nm lasers. BUV737 and BUV805 are polymer tandem dyes that are excited by the 355 nm laser and emit in the far-red spectrum.

Selection guide

DyeBUV737BUV805
Brightness
4 out of 5 (PE being the most bright)
3 out of 5 (PE being the most bright)
Excitation UV (355 nm)UV (355 nm)
Emission 737 nm805 nm
Suggested bandpass filter740/35820/60 or 780/60
More about the dyeView BUV737 pageView BUV805 page

Figure 1. Absorption and emission of (A) Brilliant Ultra Violet™ Dye 805 (BUV805) and (B) Brilliant Ultra Violet™ Dye 737 (BUV737).


Example data

Intracellular staining

Intracellular staining with BUV805 and BUV737 dyes
Dot plots of IFN-gamma-BUV805 and IFN-gamma-BUV737 vs CD4-APC and CD4-PE, respectively in unstimulated and stimulated cells

Figure 2. Flow cytometry results from intracellular staining experiment.(A) Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors; Cat. No. 00-4975-93) (right). Cells were then stained intracellularly using the Intracellular Fixation & Permeabilization Buffer Set (Cat. No. 88-8824-00) with CD4 Monoclonal Antibody, APC (Cat. No. 17-0047-42) and IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 805 (Cat. No. 368-7311-80). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Cat. No. L34964). (B) C57BL/6 mouse splenocytes were stimulated for 72 hours with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Cat. No. 16-0031-85 and Cat. No. 16-0281-86, respectively). Cells were restimulated for 5 hours with Brefeldin A (Cat. No. 00-4506-51) (left) or restimulated for 5 hours with Cell Stimulation Cocktail (Cat. No. 00-4970-93) and Brefeldin A (right). Cells were then stained intracellularly using the Intracellular Fixation & Permeabilization Buffer Set (Cat. No. 88-8824-00) with CD4 Monoclonal Antibody, PE (Cat. No. 12-0042-82) and 0.5 µg of IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 737 (Cat. No. 367-7311-80). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Cat. No. L34964).

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