The Attune NxT Flow Cytometer can be configured with up to 4 lasers to meet your needs and budget. The system’s modular design enables the system to grow with your needs via rapid field upgrades.

 

Attune NxT Flow Cytometer configuration options

Lasers Laser configuration Lasers included, No. of detection channels Total detection channels* Category Number
Violet, 405nm Blue, 488nm Yellow, 561nm Green, 532nm Red, 637nm
1 Blue
4 6 A24864
2 Blue/green
3 4 9 A28995
Blue/yellow
3 4 9 A24861
Blue/red
4 3 9 A24863
Blue/violet
4 4 10 A24862
Blue/violet 6
6 3 11 A29002
3 Blue/Green/Red
3 4 3 12 A28997
Blue/red/yellow
3 3 3 12 A28993
Blue/green/violet
4 3 4 13 A28999
Blue/violet/yellow
4 3 4 13 A24859
Blue/red/violet
4 4 3 13 A24860
Blue/red/violet 6
6 3 3 14 A29003
4 Blue/red/violet/green
4 3 4 3 16 A29001
Blue/red/yellow/violet
4 3 4 3 16 A24858
Blue/red/yellow/violet 6
6 2 2 3 16 A29004

“I would highly recommend the Attune NxT cytometer.  The best thing is that you can start with only one laser and easily field upgrade in just one afternoon, to add more in the future.”

Steve McClellan

BS, MT, SCYM (ASCP)CM

Mitchell Cancer Institute

University of South Alabama


Optics

 

Laser power
Beam shaping optics* Diode power**
   Violet: 405nm 50mW 100mW
   Blue:  488nm 50mW 100mW
   Green: 532nm 100mW 140mW
   Yellow: 561nm 50mW 100mW
   Red: 637nm 100mW 140mW
 

* Amount of measured usable laser power after light has gone through the beam optics and shaping filters.

** Vendor-specified theoretical maximum.

Laser excitation Optimized excitation for minimized stray laser-line noise and losses to reflection
Laser profile 10 μm x 50 μm flat-top laser designed for robust alignment
Emission filters Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters
Laser separation 150 μm
Optical alignment Fixed alignment with pre-aligned welded fiber; no user maintenance required
Onboard thermal-electric cooler No warm-up delay; fiber isn't affected by on/off
Simmer mode Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage
Flat top specified at the flow cell CV <3% over width of flat top
Upgradeable Convenient field changes

Fluidics

Laser profile 20 μL to 4 mL
Custom sample flow rates Custom sample flow rates
Sample delivery Positive-displacement syringe pump for volumetric analysis
Sample tubes Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
Fluid-level sensing Active
Standard fluid reservoirs 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
Fluid storage All fluids stored within instrument
Extended fluidics option Configuration for 10 L fluid
Nominal fluid consumption 1.8 L/day
Automated maintenance cycles Startup (<15 min), shutdown, rinse, wash, debubble, deep clean, monthly decontamination

Performance

Fluorescence sensitivity ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
Fluorescence resolution CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
Data acquisition rate Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
Maximum electronic speed 65,000 events/sec with all parameters
Carryover Single-tube format: <1%
Forward and side scatter sensitivity Able to discriminate platelets from noise
Forward and side scatter resolution Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole
Forward scatter Photodiode detector with 488/10 nm bandpass filter
Side scatter PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
Fluorescent detectors 14 individual detectors
Electronic pulse Measured area, height and width pulse for all detectors
Violet side scatter resolution Can be configured for violet side scatter to better resolve particles from noise
Minimum particle size 0.2 μm on side scatter using Bangs Laboratories, Inc. Submicron Bead Calibration Kit (Cat. No. 832) 0.1 μm on side scatter under following conditions: Using an Attune NxT Flow Cytometer standard 0.5 mm blocking configuration, an Attune NxT 488/10 Filter (Cat. No. 100083194) and using Attune Focusing Fluid (Cat. No. 4488621, 4449791, or A24904) that has been 0.22 µm filtered.

Software

Compensation Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
Flow rate Precise flow rate control via software; no hardware adjustments
Live streaming Live update of statistics during acquisition of events up to 35,000 events/sec
Overlays Comparative analysis between samples; 3D view
Sample recovery System able to return unused samples
Concentration Direct concentration measurement without use of counting beads
Software layout Fully customizable for each user account
Bubble detection technology Stops automated run to preserve sample integrity
Maximum single-event file 20 million with option to append
Heat map Set up for definition of plate layout; screening view for analysis for tubes and plates
Threshold Up to 4 individual thresholds with user option to apply Boolean logic
Gating Hierarchal gating with the ability to derive gates
Smartgate labeling Option to annotate quad gate names based on fluorophore and target names
Voltage User adjustable
Window extensions User adjustable
Area scaling factor (ASF) User adjustable
Acquisition settings Documented in FCS files and maintained upon import
Templates Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
Tube-to-plate conversion One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
Graphics resolution Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
User account administration Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count

Quality & regulatory

Instrument tracking Automated daily baseline and performance test with Levey-Jennings plots
Warranty 1 year
Production verification testing Each instrument is tested and verified for assembly integrity and performance to specifications
Quality management system Manufacturing standards comply with the requirements of ISO 13485:2003
Robust install specifications Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
For Research Use Only  

Data management

Software Invitrogen Attune NxT Software
Monitor 23-inch flat panel (1,920 x 1,080 resolution); dual-monitor capability
Computer Minitower desktop
Operating system Windows™ 10 software
FCS format FCS 3.1, 3.0
Processor Intel Core™ i7 processor
RAM 32 GB
Hard drives 2 x 2TB SATA 6.0Gb/s, 7200 RPM, 64MB data burst cache; Controller RAID1, integrated
Electrical requirements 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
Heat dissipation <150 W
Temperature operating ranges 15–30°C (59–86°F)
Operating humidity 10–90%, noncondensing
Audible noise <65 dBA at 1.0 m
Instrument size (HxWxD) ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
Weight ~29 kg (64 lb)

For research use only, not intended for use in diagnostic procedures.