The Attune NxT Flow Cytometer can be configured with up to 4 lasers to meet your needs and budget. The system’s modular design enables the system to grow with your needs via rapid field upgrades.
| Lasers | Laser configuration | Lasers included, No. of detection channels | Total detection channels* | Category Number | ||||
|---|---|---|---|---|---|---|---|---|
| Violet, 405nm | Blue, 488nm | Yellow, 561nm | Green, 532nm | Red, 637nm | ||||
| 1 | Blue
|
✝ | 4 | ✝ | ✝ | ✝ | 6 | A24864 |
| 2 | Blue/green
|
✝ | 3 | 4 | ✝ | 9 | A28995 | |
| Blue/yellow
|
✝ | 3 | 4 | ✝ | 9 | A24861 | ||
| Blue/red
|
✝ | 4 | ✝ | ✝ | 3 | 9 | A24863 | |
| Blue/violet
|
4 | 4 | ✝ | ✝ | ✝ | 10 | A24862 | |
| Blue/violet 6
|
6 | 3 | ✝ | ✝ | 11 | A29002 | ||
| 3 | Blue/Green/Red
|
✝ | 3 | 4 | 3 | 12 | A28997 | |
| Blue/red/yellow
|
✝ | 3 | 3 | 3 | 12 | A28993 | ||
| Blue/green/violet
|
4 | 3 | 4 | ✝ | 13 | A28999 | ||
| Blue/violet/yellow
|
4 | 3 | 4 | ✝ | 13 | A24859 | ||
| Blue/red/violet
|
4 | 4 | ✝ | ✝ | 3 | 13 | A24860 | |
| Blue/red/violet 6
|
6 | 3 | ✝ | 3 | 14 | A29003 | ||
| 4 | Blue/red/violet/green
|
4 | 3 | 4 | 3 | 16 | A29001 | |
| Blue/red/yellow/violet
|
4 | 3 | 4 | 3 | 16 | A24858 | ||
| Blue/red/yellow/violet 6
|
6 | 2 | 2 | 3 | 16 | A29004 | ||
|
*Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. ✝Laser upgrade available |
||||||||
| Laser power |
Beam shaping optics* | Diode power** |
 Violet: 405nm |
50mW | 100mW |
 Blue: 488nm |
50mW | 100mW |
 Green: 532nm |
100mW | 140mW |
 Yellow: 561nm |
50mW | 100mW |
 Red: 637nm |
100mW | 140mW |
* Amount of measured usable laser power after light has gone through the beam optics and shaping filters. ** Vendor-specified theoretical maximum. |
||
| Laser excitation | Optimized excitation for minimized stray laser-line noise and losses to reflection | |
| Laser profile | 10 μm x 50 μm flat-top laser designed for robust alignment | |
| Emission filters | Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters | |
| Laser separation | 150 μm | |
| Optical alignment | Fixed alignment with pre-aligned welded fiber; no user maintenance required | |
| Onboard thermal-electric cooler | No warm-up delay; fiber isn't affected by on/off | |
| Simmer mode | Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage | |
| Flat top specified at the flow cell | CV <3% over width of flat top | |
| Upgradeable | Convenient field changes | |
| Laser profile | 20 μL to 4 mL |
| Custom sample flow rates | Custom sample flow rates |
| Sample delivery | Positive-displacement syringe pump for volumetric analysis |
| Sample tubes | Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm |
| Fluid-level sensing | Active |
| Standard fluid reservoirs | 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank |
| Fluid storage | All fluids stored within instrument |
| Extended fluidics option | Configuration for 10 L fluid |
| Nominal fluid consumption | 1.8 L/day |
| Automated maintenance cycles | Startup (<15 min), shutdown, rinse, wash, debubble, deep clean, monthly decontamination |
| Fluorescence sensitivity | ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC |
| Fluorescence resolution | CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN) |
| Data acquisition rate | Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics |
| Maximum electronic speed | 65,000 events/sec with all parameters |
| Carryover | Single-tube format: <1% |
| Forward and side scatter sensitivity | Able to discriminate platelets from noise |
| Forward and side scatter resolution | Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole |
| Forward scatter | Photodiode detector with 488/10 nm bandpass filter |
| Side scatter | PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter |
| Fluorescent detectors | 14 individual detectors |
| Electronic pulse | Measured area, height and width pulse for all detectors |
| Violet side scatter resolution | Can be configured for violet side scatter to better resolve particles from noise |
| Minimum particle size | 0.2 μm on side scatter using Bangs Laboratories, Inc. Submicron Bead Calibration Kit (Cat. No. 832) 0.1 μm on side scatter under following conditions: Using an Attune NxT Flow Cytometer standard 0.5 mm blocking configuration, an Attune NxT 488/10 Filter (Cat. No. 100083194) and using Attune Focusing Fluid (Cat. No. 4488621, 4449791, or A24904) that has been 0.22 µm filtered. |
| Compensation | Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells |
| Flow rate | Precise flow rate control via software; no hardware adjustments |
| Live streaming | Live update of statistics during acquisition of events up to 35,000 events/sec |
| Overlays | Comparative analysis between samples; 3D view |
| Sample recovery | System able to return unused samples |
| Concentration | Direct concentration measurement without use of counting beads |
| Software layout | Fully customizable for each user account |
| Bubble detection technology | Stops automated run to preserve sample integrity |
| Maximum single-event file | 20 million with option to append |
| Heat map | Set up for definition of plate layout; screening view for analysis for tubes and plates |
| Threshold | Up to 4 individual thresholds with user option to apply Boolean logic |
| Gating | Hierarchal gating with the ability to derive gates |
| Smartgate labeling | Option to annotate quad gate names based on fluorophore and target names |
| Voltage | User adjustable |
| Window extensions | User adjustable |
| Area scaling factor (ASF) | User adjustable |
| Acquisition settings | Documented in FCS files and maintained upon import |
| Templates | Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously |
| Tube-to-plate conversion | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes |
| Graphics resolution | Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software) |
| User account administration | Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count |
| Instrument tracking | Automated daily baseline and performance test with Levey-Jennings plots |
| Warranty | 1 year |
| Production verification testing | Each instrument is tested and verified for assembly integrity and performance to specifications |
| Quality management system | Manufacturing standards comply with the requirements of ISO 13485:2003 |
| Robust install specifications | Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure |
| For Research Use Only |
| Software | Invitrogen Attune NxT Software |
| Monitor | 23-inch flat panel (1,920 x 1,080 resolution); dual-monitor capability |
| Computer | Minitower desktop |
| Operating system | Windows™ 10 software |
| FCS format | FCS 3.1, 3.0 |
| Processor | Intel Core™ i7 processor |
| RAM | 32 GB |
| Hard drives | 2 x 2TB SATA 6.0Gb/s, 7200 RPM, 64MB data burst cache; Controller RAID1, integrated |
| Electrical requirements | 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825. |
| Heat dissipation | <150 W |
| Temperature operating ranges | 15–30°C (59–86°F) |
| Operating humidity | 10–90%, noncondensing |
| Audible noise | <65 dBA at 1.0 m |
| Instrument size (HxWxD) | ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles |
| Weight | ~29 kg (64 lb) |
For research use only, not intended for use in diagnostic procedures.
