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Flow Cytometry Assays and Reagents
Cell functional assays: dyes and reagents
Simultaneously analyze multiple proteins, gene expression, and cell functions such as oxidation, viability, cell cycle, apoptosis, and proliferation from an individual cell with fluorescent flow cytometry assays, reagents, and antibodies. This technology makes it possible to obtain a statistically relevant amount of data by combining information from individual cells to gain insight into a heterogeneous sample. We offer flow cytometry assays and reagents that span a broad range of research applications and areas including cell health and function assays as well as RNA detection assays.
Trying to incorporate a reagent into your panel?Ask our technical specialists which you should purchase and how to incorporate into your experiment
FREE: Flow Cytometry Panel Design Service and Panel Builder Tool
For a personalized panel design service, contact our flow cytometry panel design team.
Our flow cytometry scientists work with you to develop optimized panels (conventional and spectral) to fit your experiment and your instrument. You can use our panel design service as many times as you need—free of charge, and all panels are reviewed by at least 2 experts.
We also offer a free, do-it-yourself (DIY) webtool—Flow Cytometry Panel Builder Tool. This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Need to add a flow cytometer to your lab?Learn more about the Invitrogen Attune NxT flow cytometer here
Click image to enlarge
Figure 1. Cell function workflow with flow cytometry reagents. Use the chart to determine which assays can be incorporated into a panel. Fluorophore reagents can be used to label cell functionalities such as viability and mitochondrial oxidation. These reagents and assays can be incorporated into a flow cytometry panel just like a flow cytometry antibody.
Flow cytometry assays and reagents selections
Cell viability assays
Distinguishing dead cells and removing artifacts in your assay is a critical step to help ensure accurate results.
RNA assays
Simultaneous detection of RNA and proteins by flow cytometry using the PrimeFlow RNA Assay. Combine the PrimeFlow assay with immunolabeling of both cell-surface and intracellular proteins.
Cell proliferation assays
Measuring cell proliferation is a fundamental method for assessing cell health and cell division.
Cell cycle assays
We offer a series of fluorescent reagents designed for accurate cell cycle analysis in either live- or fixed-cell populations.
Metabolism and oxidative stress assays
Using flow cytometry, you can measure mitochondrial membrane potential in live cells, label mitochondria in live cells, and measure generalized oxidative stress in cells with fluorogenic probes.
Microbiology assays
Assess key metabolic processes and monitor bacteria/yeast growth, proliferation, and vitality.
Senescence
Fluorescent detection of β-galactosidase hydrolysis by flow cytometry.
Apoptosis assays
Cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach to apoptosis detection.
Ready-to-use flow cytometry reagents
Invitrogen Ready Flow reagents are room temperature-stable, ready-to-use solutions designed to allow you to stain your cells for analysis by flow cytometry.
Immunophenotyping kits
The Invitrogen eBioscience essential phenotyping kits are pre-assembled multicolor panels designed to detect immune cells by flow cytometry.
Flow cytometry beads and buffers
Find tools for cell concentration and absolute counting, as well as distinguishing and quantitating live and dead bacteria, setting up compensation for fluorescent reagents.
Try sample preparation buffers to simplify your flow cytometry experiments. Compatible with fluorescent reagents.
Immunology at Work
Learn immune cell type markers and protocols. The Invitrogen Immunology at Work Resource Center is a learning center with technical content designed for new and experienced life scientists alike exploring the field of immunology.
5 Steps to Intracellular Flow Cytometry
Discover products and resources that are available to help optimize your intracellular flow cytometry experiments.
Resources
Fluorophore and reagent selection guide for flow cytometry
Download Flow Cytometry Protocols Handbook
Spectral Flow Cytometry Fundamentals
Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.
Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.
Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.
Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.
5 Steps Resources
Support
Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.
Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.