Graphic of red flow cytometry beads

Why use a bead standard?

Flow cytometry is used to obtain quantifiable results in reference to the physical characteristics of single cells. Microspheres are small, non-biological beads that can be used as a (1) standard for flow cytometer instrument calibration, (2) reference for cell or particle size, and (3) experimental control for fluorescence emission spillover. These beads are designed to provide:

  • Consistent instrument performance with minimal data variation for alignment, size calibration, and sorting set-up.
  • Convenience to save sample and set compensation for antibody, reagent, and fluorescent proteins.
  • Comparable data between experiments and instruments by setting a standard.


Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.

5 Steps Resources


Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Protocols

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