Graphic of red flow cytometry beads

Why use a bead standard?

Flow cytometry is used to obtain quantifiable results in reference to the physical characteristics of single cells. Microspheres are small, non-biological beads that can be used as a (1) standard for flow cytometer instrument calibration, (2) reference for cell or particle size, and (3) experimental control for fluorescence emission spillover. These beads are designed to provide:

  • Consistent instrument performance with minimal data variation for alignment, size calibration, and sorting set-up.
  • Convenience to save sample and set compensation for antibody, reagent, and fluorescent proteins.
  • Comparable data between experiments and instruments by setting a standard.


Fluorophore and reagent selection guide for flow cytometry

Download Flow Cytometry Protocols Handbook

Spectral Flow Cytometry Fundamentals

Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.

Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.

Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.

5 Steps Resources


Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.

Flow Cytometry Protocols

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