Why use a bead standard?
Flow cytometry is used to obtain quantifiable results in reference to the physical characteristics of single cells. Microspheres are small, non-biological beads that can be used as a (1) standard for flow cytometer instrument calibration, (2) reference for cell or particle size, and (3) experimental control for fluorescence emission spillover. These beads are designed to provide:
- Consistent instrument performance with minimal data variation for alignment, size calibration, and sorting set-up.
- Convenience to save sample and set compensation for antibody, reagent, and fluorescent proteins.
- Comparable data between experiments and instruments by setting a standard.
Compensation beads are used as single color controls and can be an alternative for stained single cell controls. Learn about the different beads for multicolor flow cytometry experiments including antibodies, fluorescent proteins, and reagents.
To help ensure collection of reproducible and accurate results, flow cytometer parameters can be set and monitored through the use of standardized beads. Understand the different beads required for flow cytometry sorting parameters, instrument set-up, and daily performance.
Cell counting beads are designed to provide a known quantity of microspheres per known volume. Find beads for cell concentration and absolute counting for mammalian cells, particles, and bacteria.
Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.
Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.
Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.
Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.
5 Steps Resources
Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.
Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.