Flow Cytometry Learning Center

Spectral Flow & Flow Protocols handbooks

Protocols, practical insights, application data and more.

Download both handbooks

Fluorophore and reagent selection guides

Recommended flow cytometry antibodies and reagents for flow cytometers: Download guides for Attune Xenith , BD FACSDiscover™ S8 Sorter, Cytek™ Aurora/CS.

Flow cytometry monthly webinar series

Let's Talk Flow is a bite-sized educational platform with exclusive face-to-face time with industry leaders, helping you enhance your flow cytometry knowledge and skills.

Register now

Molecular Probes School of Fluorescence

The Molecular Probes School of Fluorescence was developed by our in-house bench scientists, featuring everything we wish we'd known when we first started working with fluorescent reagents, including the basics of fluorescence, imaging, and flow cytometry.

eLearning Courses

Our eLearning courses were developed to help provide a succinct, contextual summary of common laboratory techniques and methods only used by researchers with an interest in protein and cell analysis workflows.

• T Cell Stimulation and Proliferation eLearning Course

FREE: Flow Cytometry Panel Design Service and Panel Builder Tool

For a personalized panel design service, contact our flow cytometry panel design team. Our flow cytometry scientists work with you to develop optimized panels (conventional and spectral) to fit your experiment and your instrument. You can use our panel design service as many times as you need—free of charge, and all panels are reviewed by at least 2 experts.

We also offer a free, do-it-yourself (DIY) webtool—Flow Cytometry Panel Builder Tool. This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.

Areas of biology information

Flow cytometry is a powerful tool in the study of many areas of biology. In this subtopic section you will find educational assets for help with immunology, immuno-oncology, cancer, stem cell research and more.

Cell isolation, expansion and differentiation information

Cell isolation, expansion and differentiation are critical processes and techniques for studying cell biology. Here you will find educational resources including videos, application notes and articles to help you better understand these processes.

Controls, compensation and calibration information

Controls, compensation and calibration are all critical to obtaining accurate results in flow cytometric analysis. In this section you will find educational resources to help you set up your flow cytometer and analyze your samples.

Flow cytometry assays information

Learn about assays and techniques for detecting and monitoring cell health and function by flow cytometry. Topics include apoptosis, cell counting, proliferation and viability as well as cell cycle, endocytosis, oxidative stress and more.

Flow cytometry instrumentation information

These resources will help you increase your understanding of the fundamentals and scientific principles of flow cytometry, and expand your abilities for specialty applications and techniques.

Immunophenotyping information

This section provides educational resources and tools for immunophenotyping by flow cytometry.