Apoptosis is a naturally occurring process by which a cell is directed to programmed cell death. In this section you will find educational resources to help you design and execute experiments including application notes, scientific posters, webinars, and signaling pathways.
Find information about the numerous and complex signaling pathways involved in the process of apoptosis including pathway schematics and overviews.
This journal club provides a review of a recently published methods chapter in Current Protocols by Telford and coauthors that offers detailed protocols for several methods to measure and characterize apoptosis using multiparametric flow cytometry. The article provides basic theory discussions, detailed reagent listings, step-by-step procedures, and guidance on analysis methods, while also identifying critical items to consider when performing these assays.
Apoptosis learning resources
|Application note (2017)||Flow cytometry analysis of dose response for apoptosis induction||apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green|
|BioProbes article (2017)||Journal club: measurement and characterization of apoptosis by flow cytometry||apoptosis, caspase substrates, flow cytometry, fluorescence, viability|
|BioProbes article (2015)||Journal Club—Multiparameter detection of early apoptosis markers without compensation||apoptosis, Attune NxT, cell structure-mitochondria, flow cytometry compensation, fluorescence, immunophenotyping|
|BioProbes article (2010)||A ratiometric approach to apoptosis detection by flow cytometry||apoptosis, flow cytometry, fluorescence|
|BioProbes article (2010)||Advanced cell proliferation analysis||Attune NxT cell proliferation, cell tracking, flow cytometry, fluorescence|
|BioProbes article (2009)||A growing list of tools for the violet laser||Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability|
|BioProbes article (2007)||Investigating apoptosis-induced loss of SYTO dye fluorescence||apoptosis, cell structure-mitochondria, enzyme activity detection, flow cytometry, fluorescent dyes|
|BioProbes article (2007)||Looking for apoptosis?||antibodies, apoptosis, colorimetric, ELISA, flow cytometry, fluorescence, fluorescent dyes, immunocytochemistry (ICC), protein detection, western blotting|
|BioProbes article (2007)||Multiparametric approaches for studying apoptosis||Alexa Fluor, antibodies, apoptosis, cell structure-mitochondria, cell structure-plasma membrane, enzyme activity detection, flow cytometry, fluorescent dyes, viability|
|BioProbes article (2006)||Analyzing cell culture health and concentration||apoptosis, cell counting, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability|
|Molecular Probes Handbook||Assays for apoptosis and autophagy—Section 15.5||apoptosis, autophagy, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging|
|Protocols||Annexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents)||apoptosis, flow cytometry|
|Protocols||Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)||apoptosis, flow cytometry|
|Scientific poster (2018)||Fluorescent hypoxia sensor for flow cytometry||apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology|
|Scientific poster (2013)||Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent||Alexa Fluor, apoptosis, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry|
|Scientific poster (2010)||Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry||apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress|
|Scientific poster (2009)||A simple, fast and quantitative single-step dead-cell indicator for flow cytometry||apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability|
|Scientific poster (2006)||Novel violet-excited reagents for detection of viability and vitality||Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents|
|CYToMORE lecture video (2016)||Novel reagent solutions for flow cytometry |
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
|Attune NxT, flow cytometry assays|
|Webinar||An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis |
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
|apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability|
For Research Use Only. Not for use in diagnostic procedures.