Invitrogen eBioscience Super Bright 436 antibody conjugates for flow cytometry provide:
Figure 1. Super Bright 436 dye performance comparison. Mouse bone marrow cells were stained with Anti-Ly-6A/E (clone D7) APC and either Anti-CD117 (clone 2B8) conjugated to (left panel) Super Bright 436 dye, (middle panel) eFluor 450 dye or (right panel) Brilliant Violet™ 421 dye.
Figure 2. Fluorescence intensity for Super Bright 436 dye conjugates compared to Brilliant Violet™ 421 conjugates. (A) Human peripheral blood cells were stained with CD19 antibodyconjugated to either Super Bright 436 (purple, Cat. No. 62-0199-42), eFluor 450 dye (blue, Cat. No.48-0199-42), or Brilliant Violet™ 421 dye (orange). (B) Human peripheral blood cells were stained withCD27 antibody conjugated to either Super Bright 436 (purple, Cat. No. 62-0279-42) or Brilliant Violet™421 dye (orange).
|Multiplexing compatibility||Buffer||Multiplexing considerations|
|Multiplexing with 1 Super Bright antibody conjugate||Standard buffers applicable||No special buffer required when only one Super Bright antibody conjugate is used in a panel|
|Multiplexing with 2 or more Super Bright antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions|
|Multiplexing with 1 or more Brilliant Violet™ antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted|
Figure 3. 10-color ILC2 subset panel. Normal human PBMCs were surface-stained in the presence of Super Bright Staining Buffer (Cat. No. SB-4400-42) at optimal concentrations for the indicated surface markers.(A) Gated on live cells, this plot shows the lineage markers used as a FITC dump channel (CD3 (clone UCHT1), CD4 (clone SK3), CD8a (clone RPA-T8), CD11b (clone ICRF44), and CD19 (clone HIB19) vs. CD127 (IL-7RA) (clone eBioRDR5) Super Bright 436 (Cat. No. 62-1278-42) stained cells. Since ILC2 subsets are negative for all ﬁve of these markers, all CD127 and lineage-positive cells can be eliminated from further analysis. (B-G) Gating strategy is shown for all lineage targets to highlight the ILC2 population. (H, I) The CD294 (CRTH2) population is identiﬁed.
|Viability stain options||Product||Multiplexing considerations|
|Fixable||LIVE/DEAD fixable dead cell stain kits||Not compatible with LIVE/DEAD Fixable Violet Dead Cell Stain or Fixable Viability Dye eFluor 450|
|Non-fixable||SYTOX non-fixable dead cell stains|
Ready Flow Ready-to-use viability reagents
|Not compatible with SYTOX blue stain|
Compatible with all Ready Flow reagents for viability
|UltraComp eBeads microspheres||UltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates.|
|Staining Target||Product||Multiplexing considerations|
|Intracellular Fixation & Permeabilization Buffer Set||No compatibility concerns|
|Foxp3/Transcription Factor Staining Buffer Set||No compatibility concerns|
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.