Comparable to Brilliant Violet 605 conjugates
Invitrogen eBioscience Super Bright 600 antibody conjugates for flow cytometry provide:
- Brightness and resolution of positive and negative populations comparable to that of Brilliant Violet™ 605 conjugates
- Options in marker and clone selection for violet laser excitable antibody conjugates
- Compatibility with standard intracellular buffers, viability stains, compensation beads, and other antibodies
Multiplexing with Super Bright 600 antibody conjugates
Figure 1. Staining performance and post-fixation stability. (A) Comparison of mouse splenocytes stained with CD8a antibody conjugated to either Super Bright 600 (red, Cat. No. 63-0081-82) or Brilliant Violet™ 605 conjugate (gray), at the same antibody concentration. (B) Mouse splenocytes were stained with CD45R antibody conjugated to Super Bright 600 (Cat. No. 63-0452-82) and either left unfixed (red), or were fixed in Invitrogen eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) for 30 minutes (blue), 24 hours (orange), or 3 days (green).
|Multiplexing compatibility||Buffer||Multiplexing considerations|
|Multiplexing with 1 Super Bright antibody conjugate||Standard buffers applicable||No special buffer required when only one Super Bright antibody conjugate is used in a panel|
|Multiplexing with 2 or more Super Bright antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions|
|Multiplexing with 1 or more Brilliant Violet™ antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted|
|Viability stain options||Product||Multiplexing considerations|
|Fixable||LIVE/DEAD fixable dead cell stain kits||No compatibility concerns|
|Non-fixable||SYTOX non-fixable dead cell stains
Ready Flow Ready-to-use viability reagents
|No compatibility concerns
Compatible with all Ready Flow reagents for viability
|Staining Target||Product||Multiplexing considerations|
|Intracellular Fixation & Permeabilization Buffer Set||No compatibility concerns|
|Foxp3/Transcription Factor Staining Buffer Set||No compatibility concerns
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.