Invitrogen eBioscience Super Bright 645 antibody conjugates for flow cytometry provide:
Figure 1. Fluorescence intensity comparison of Super Bright 645 conjugates and Brilliant Violet™ 650 conjugates.(A) Mouse splenocytes stained with anti-CD8a conjugated to Super Bright 645 (red, Cat. No. 64-0081-82) or Brilliant Violet™ 650 conjugate (gray), at the same concentration of antibody. (B) Human peripheral blood cells stained with anti-CD8a conjugated to Super Bright 645 (red, Cat. No. 64-0088-42) or Brilliant Violet™ 650 conjugate (gray), using the same concentration of antibody.
|Multiplexing compatibility||Buffer||Multiplexing considerations|
|Multiplexing with 1 Super Bright antibody conjugate||Standard buffers applicable||No special buffer required when only one Super Bright antibody conjugate is used in a panel|
|Multiplexing with 2 or more Super Bright antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions|
|Multiplexing with 1 or more Brilliant Violet™ antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted|
|Viability stain options||Product||Multiplexing considerations|
|Fixable||LIVE/DEAD fixable dead cell stain kits||No compatibility concerns|
|Non-fixable||SYTOX non-fixable dead cell stains|
Ready Flow Ready-to-use viability reagents
|No compatibility concerns|
Compatible with all Ready Flow reagents for viability
|UltraComp eBeads microspheres||UltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates.|
|Staining Target||Product||Multiplexing considerations|
|Intracellular Fixation & Permeabilization Buffer Set||No compatibility concerns|
|Foxp3/Transcription Factor Staining Buffer Set||No compatibility concerns|
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.