Invitrogen eBioscience Super Bright 702 antibody conjugates for flow cytometry provide:
Figure 1. Fluorescence intensity comparison of Super Bright 702 conjugates to Brilliant Violet™ 711 conjugates.(A) Mouse splenocytes stained with anti-CD4 conjugated to Super Bright 702 (red) or Brilliant Violet™ 711 conjugate (gray), at the same concentration of antibody. (B) Human peripheral blood cells stained with anti-CD19 conjugated to Super Bright 702 (red) or Brilliant Violet 711™ conjugate (gray), using the same concentration of antibody.
|Multiplexing compatibility||Buffer||Multiplexing considerations|
|Multiplexing with 1 Super Bright antibody conjugate||Standard buffers applicable||No special buffer required when only one Super Bright antibody conjugate is used in a panel|
|Multiplexing with 2 or more Super Bright antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions|
|Multiplexing with 1 or more Brilliant Violet™ antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted|
Figure 2. 10-color ILC2 subset panel. Normal human PBMCs were surface-stained in the presence of Super Bright Staining Buffer (Cat. No. SB-4400-42) at optimal concentrations for the indicated surface markers.(A) Gated on live cells, this plot shows the lineage markers used as a FITC dump channel (CD3 (clone UCHT1), CD4 (clone SK3), CD8a (clone RPA-T8), CD11b (clone ICRF44), and CD19 (clone HIB19) vs. CD127 (IL-7RA) (clone eBioRDR5) Super Bright 436 (Cat. No. 62-1278-42) stained cells. Since ILC2 subsets are negative for all ﬁve of these markers, all CD127 and lineage-positive cells can be eliminated from further analysis. (B-G) Gating strategy is shown for all lineage targets to highlight the ILC2 population. (H, I) The CD294 (CRTH2) population is identiﬁed.
|Viability stain options||Product||Multiplexing considerations|
|Fixable||LIVE/DEAD fixable dead cell stain kits||No compatibility concerns|
|Non-fixable||SYTOX non-fixable dead cell stains|
Ready Flow Ready-to-use viability reagents
|No compatibility concerns|
Compatible with all Ready Flow reagents for viability
|UltraComp eBeads microspheres||UltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates.|
|Staining Target||Product||Multiplexing considerations|
|Intracellular Fixation & Permeabilization Buffer Set||No compatibility concerns|
|Foxp3/Transcription Factor Staining Buffer Set||No compatibility concerns|
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.