Invitrogen eBioscience Super Bright 780 antibody conjugates for flow cytometry provide:
Figure 1. Fluorescence intensity comparison of Super Bright 780 conjugates and Brilliant Violet™ 786 conjugates. (A) Mouse splenocytes stained with anti-CD4 conjugated to Super Bright 780 (red, Cat. No. 78-0042-80) or Brilliant Violet 786 conjugate (gray), at the same concentration of antibody. (B) Human peripheral blood cells stained with anti-CD8a conjugated to Super Bright 780 (red, Cat. No. 78-0088-41) or Brilliant Violet™ 786 conjugate (gray), using the same concentration of antibody.
Figure 2. Fluorescence intensity comparison of Super Bright 780 conjugates and Brilliant Violet™ 786 conjugates. Human peripheral blood cells stained with anti-CD19 conjugated to (A) Brilliant Violet™ 786 or (B) Super Bright 780 (Cat. No. 78-0198-41) at the same concentration of antibody.
Figure 3. Fluorescence intensity comparison of Super Bright 780 conjugates to eFluor 450 and PE conjugates. Mouse splenocytes cells stained with anti-CD3e conjugated to (A) eFluor 450 (Cat. No. 48-0621-80), (B) PE (Cat. No. 12-0621-81) or (C) Super Bright 780 (Cat. No. 78-0621-80).
|Multiplexing compatibility||Buffer||Multiplexing considerations|
|Multiplexing with 1 Super Bright antibody conjugate||Standard buffers applicable||No special buffer required when only one Super Bright antibody conjugate is used in a panel|
|Multiplexing with 2 or more Super Bright antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions|
|Multiplexing with 1 or more Brilliant Violet™ antibody conjugates||Super Bright Staining Buffer||Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted|
|Viability stain options||Product||Multiplexing considerations|
|Fixable||LIVE/DEAD fixable dead cell stain kits||No compatibility concerns|
|Non-fixable||SYTOX non-fixable dead cell stains|
Ready Flow Ready-to-use viability reagents
|No compatibility concerns|
Compatible with all Ready Flow reagents for viability
|UltraComp eBeads microspheres||UltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates.|
|Staining Target||Product||Multiplexing considerations|
|Intracellular Fixation & Permeabilization Buffer Set||No compatibility concerns|
|Foxp3/Transcription Factor Staining Buffer Set||No compatibility concerns|
Figure 4. Staining performance and post-fixation stability. Human peripheral blood cells stained with CD8a antibody conjugated to Super Bright 780 (Cat. No. 78-0088-41) and analyzed immediately (red) or subjected to different fixation buffers per the recommended protocol: (A) eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00), (B) eBioscience IC Fixation Buffer (Cat. No. 00-8222-49), or (C) eBioscience IC Fixation Buffer followed by 30 minutes in 90% methanol and were analyzed at 30 min (blue), overnight (orange) or after 3 days (green).
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.