Alexa Fluor® 568 dye is a bright, orange-fluorescent dye with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. For stable signal generation in imaging and flow cytometry, Alexa Fluor® 568 dye is pH-insensitive over a wide molar range.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor® 568 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor® 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Alexa Fluor® 568 dye dashboard
Bright and photostable orange-red dye that matches well with RFP filter sets. In addition, is used in stochastic optical reconstruction microscopy (STORM)—as a reporter in dSTORM.
Photostability in buffer
Photostability in antifade
Performance in SRM
Alexa Fluor® 568 products
We offer a broad range of Alexa Fluor® 568 products including labeling kits, secondary antibodies, bioconjugates, and more:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits for direct detection
- Secondary antibodies for flexible labeling
- Streptavidin conjugates for signal amplification
- Tyramide labeling kits for ultimate sensitivity
- Phalloidin conjugates for actin staining
- Annexin V conjugates for apoptosis imaging
- Hydrazides for neuronal tracing
- Labeled nucleotides for hybridization probes
Fixed and permeabilized muntjac skin fibroblasts stained with Alexa Fluor® 488 phalloidin, an anti–α-tubulin antibody, and an anti–cdc6 peptide antibody. The anti–α-tubulin antibody was prelabeled with the Zenon® Alexa Fluor® 568 Mouse IgG1 Labeling Kit, and the anti–cdc6 peptide antibody was prelabeled with the Zenon® Alexa Fluor® 350 Mouse IgG1 Labeling Kit.
Alexa Fluor® 568 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
|Amine||Thiol||Aldehyde, ketone, carboxylate, or phosphate|
Alexa Fluor® 568 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon® Alexa Fluor® 568 Mouse IgG1 Labeling Kit||Z25006|
|<1–20 µg mouse IgG2a||Zenon® Alexa Fluor® 568 Mouse IgG2a Labeling Kit||Z25106|
|<1–20 µg rabbit IgG||Zenon® Alexa Fluor® 568 Rabbit IgG Labeling Kit||Z25306|
|Conventional antibody/protein labeling|
|10–20 µg antibody||APEX™ Alexa Fluor® 568 Antibody Labeling Kit||A10494|
|100 µg antibody/protein||Alexa Fluor® 568 Antibody/Protein Labeling Kit||A20184|
|1 mg antibody/protein||Alexa Fluor® 568 Antibody/Protein Labeling Kit||A10238|
Alexa Fluor® dye family
Illuminating research for over 15 years
Alexa Fluor® dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor® dyes.
- Learn more about the brightness of Alexa Fluor® dyes
- Learn more about the photostability of Alexa Fluor® dyes
- Read all about Alexa Fluor® dyes in the Molecular Probes® Handbook