Alexa Fluor® 594 dyeInvitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 594 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

We offer Alexa Fluor 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor 594 dye dashboard


Initial brightness
 
 
 
 
 
Direct replacement for Invitrogen Texas Red dye with bright signal and excellent photostability, especially in fixed cells; commonly used with Invitrogen Alexa Fluor 350, 488, and 647 dyes for multiplexing. In addition, is used in a range of SRM applications including dSTORM, SIM, and STED and in two-photon excitation (TPE) microscopy. 
  

Photostability in buffer
 
 
 
 
 
  

Photostability in antifade
 
 
 
 
 
  

Performance in SRM
 
 
 
 
 
 561/594Texas Red590617 
 
Laser line
Common filter set
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Emission max

 

Alexa Fluor® 594 dye

Microtubules of fixed bovine pulmonary artery endothelial cells were visualized with mouse monoclonal anti–α-tubulin antibody, followed by an Alexa Fluor 350 goat anti–mouse IgG antibody. Next, the F-actin was labeled with Alexa Fluor 594 phalloidin. Finally, the cells were incubated with Alexa Fluor 488 wheat germ agglutinin to stain components of endosomal pathways. The superimposed and pseudocolored images were acquired sequentially using bandpass filter sets appropriate for DAPI, Texas Red dye, and fluorescein, respectively.

Alexa Fluor 594 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

AmineThiolAldehyde, ketone, carboxylate, or phosphateClick reaction

Alexa Fluor 594 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target proteinKitCat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1Zenon Alexa Fluor 594 Mouse IgG1 Labeling KitZ25007
<1–20 µg mouse IgG2aZenon Alexa Fluor 594 Mouse IgG2a Labeling KitZ25107
<1–20 µg mouse IgG2bZenon Alexa Fluor 594 Mouse IgG2b Labeling KitZ25207
<1–20 µg human IgGZenon Alexa Fluor 594 Human IgG Labeling KitZ25407
<1–20 µg rabbit IgGZenon Alexa Fluor 594 Rabbit IgG Labeling KitZ25307
Conventional antibody/protein labeling
10–20 µg antibodyAPEX Alexa Fluor 594 Antibody Labeling KitA10474
20–100 µg antibody/proteinAlexa Fluor 594 Microscale Antibody/Protein Labeling KitA30008
100 µg antibody/proteinAlexa Fluor 594 Antibody/Protein Labeling KitA20185
1 mg antibody/proteinAlexa Fluor 594 Antibody/Protein Labeling KitA10239
Alexa Fluor Dye stained cell

Alexa Fluor dye family

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Learn more about the:

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.