Alexa Fluor 594 excitation shown as dotted line and emission shown as solid orange histogram
Brightness iconLaser line iconCommon filter set iconExcitation iconEmission iconPhotostability iconInstrument platform icon
5561/594Texas Red590618(in buffer) 3
(in antifade) 4
microscopy; flow cytometry; fluorescence microplate reader

Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. It is commonly used with Alexa Fluor 350, Alexa Fluor 488 and Alexa Fluor 647 dyes for multiplexing. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. In addition, it is used in a range of SRM applications including dSTORM, structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy and in two-photon excitation (TPE) microscopy. 

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 594 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

We offer Alexa Fluor 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available that allows you to generate your own antibody conjugates or probes.

Search Alexa Fluor 594 secondary antibodies Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)

Microscopic view of bovine endothelial cells with F-actin (orange), alpha-tubulin (blue) and endosomal pathway components (green)

Fluorescence microscopy of bovine endothelial cells. Microtubules of fixed bovine pulmonary artery endothelial cells localized with alpha-Tubulin Monoclonal Antibody , which was subsequently visualized with Goat anti-Mouse IgG Cross-Adsorbed Antibody, Alexa Fluor 350. Next, the F-actin was labeled with Alexa Fluor 594 Phalloidin. Finally, the cells were incubated with Wheat Germ Agglutinin, Alexa Fluor 488 Conjugate to stain components of endosomal pathways. The superimposed and pseudocolored images were acquired sequentially using bandpass filter sets appropriate for DAPI, the Texas Red dye and FITC, respectively.

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