graph of emission spectra for APC-eFluor 780 dye

APC-eFluor 780 organic dye was developed to provide a high performance tandem fluorophore for multicolor flow cytometry. It was designed for use with flow cytometry instruments equipped with a red laser and has an emission peak of 780 nm. With similar spectral characteristics to APC-Alexa Fluor 750, APC-eFluor 780 requires no changes to your instrument configuration for use, allowing you to easily add APC-eFluor 780 to your multicolor panels.

Benefits of APC-eFluor 780 include

  • Equal or better sensitivity than APC-H7 tandem conjugates
  • Stable performance when treated with formaldehyde fixation or exposed to ambient light
  • Uses same filter sets as existing comparative fluorochromes

APC-eFluor 780 dye dashboard


Initial brightness

 
 
 
 
 
APC-eFluor 780 dye has similar spectral properties to APC-H7, APC-Cyanine7 and APC-Alexa Fluor 750 dye. It can replace any of these tandem dyes when designing a multicolor staining panel. The APC-eFluor 780 tandem dye is easily compensated when used with APC.  
   

Photostability in buffer

 
 
 
 
 

  633 780/60 655 780  
 
Laser line

Common filter set

Excitation max

Emission max
 
Flow histogram comparing APC-eFluor 780 dye to APC-H7

Comparison with APC-H7. Mouse splenocytes stained with Anti-CD8 (clone 53-6.7) conjugated to APC-eFluor 780 (blue line) or APC-H7 (red line).

 

Flow histogram comparing APC-eFluor dye 780 before and after aldehyde fixation
Stability of APC-eFluor 780. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-CD8 APC-eFluor 780 (clone RPA-T8) and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde for 30 minutes at room temperature (green line) and analyzed again.

APC-eFluor 780 dye products

We offer APC-eFluor 780 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

See all APC-eFluor 780 Primary Antibodies

Resources

Primary antibodies to match your cytometer's lasers

Cell stain tool

To support your multicolor flow cytometry experiments, our antibody portfolios include a variety of dyes with compatibility to all commonly used flow cytometry lasers.

Fluorescence SpectraViewer

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Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.