graph of emission spectra for APC-eFluor 780 dye

APC-eFluor 780 organic dye was developed to provide a high performance tandem fluorophore for multicolor flow cytometry. It was designed for use with flow cytometry instruments equipped with a red laser and has an emission peak of 780 nm. With similar spectral characteristics to APC-Alexa Fluor 750, APC-eFluor 780 requires no changes to your instrument configuration for use, allowing you to easily add APC-eFluor 780 to your multicolor panels.

Benefits of APC-eFluor 780 include

  • Equal or better sensitivity than APC-H7 tandem conjugates
  • Stable performance when treated with formaldehyde fixation or exposed to ambient light
  • Uses same filter sets as existing comparative fluorochromes

APC-eFluor 780 dye dashboard


Initial brightness

 
 
 
 
 
APC-eFluor 780 dye has similar spectral properties to APC-H7, APC-Cyanine7 and APC-Alexa Fluor 750 dye. It can replace any of these tandem dyes when designing a multicolor staining panel. The APC-eFluor 780 tandem dye is easily compensated when used with APC.  
   

Photostability in buffer

 
 
 
 
 

  633 780/60 655 780  
 
Laser line

Common filter set

Excitation max

Emission max
 
Flow histogram comparing APC-eFluor 780 dye to APC-H7

Comparison with APC-H7. Mouse splenocytes stained with Anti-CD8 (clone 53-6.7) conjugated to APC-eFluor 780 (blue line) or APC-H7 (red line).

 

Flow histogram comparing APC-eFluor dye 780 before and after aldehyde fixation
Stability of APC-eFluor 780. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-CD8 APC-eFluor 780 (clone RPA-T8) and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde for 30 minutes at room temperature (green line) and analyzed again.

APC-eFluor 780 dye products

We offer APC-eFluor 780 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

See all APC-eFluor 780 Primary Antibodies

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.