Spectraviewer image of Brilliant Ultra Violet 395 dye

Brilliant Ultra Violet™ dyes, including Brilliant Ultra Violet™ 395 (BUV395) dye, are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.

BUV395 is a dye excited by the 350 nm UV laser and emits at 395 nm. This dye has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend that you use Super Bright Complete Staining Buffer (Cat. No. SB-4401) or Brilliant Stain Buffer (Cat. No. 00-4409-42) to minimize any non-specific polymer interactions.

BUV395 dye dashboard

Initial brightness

The UV laser-excitable BUV395 dye is optimized for use for both cell surface and intracellular flow applications. Can be used for spectral flow cytometry applications.  

Photostability in buffer


Laser line


Excitation max

Emission max


BUV395 dye products

We offer BUV395 dye conjugated to primary antibodies for use in flow cytometry.

Antibody conjugates

Brilliant Stain Buffer
Super Bright Staining Buffer

Experimental data using BUV395 dye products

Figure 1. Excitation and emission of Brilliant Ultra Violet™ 395 dye (BUV395).

Figure 2. Spectral signature of BUV395 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with clone SK7 (hCD3) conjugated to BUV395 dye (Cat. No. 363-0036-42) were used for analysis.

Dot plot of IFN-gamma BUV395 vs CD4 FITC in unstimulated and stimulated cells

Figure 3. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Cat. No. 00-4975-03) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Cat. No. 88-8824-00) and protocol, with CD4 Monoclonal Antibody, FITC (Cat. No. 11-0049-42) and IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 395 dye (Cat. No. 363-7319-42). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50).

Dot plot of samples stained with IgG1 control vs CD19-FITC and CD3-BUV395 vs CD19-FITC

Figure 4. Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Cat. No. 11-0199-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 395 dye (Cat. No. 363-4714-81) (left) or CD3 Monoclonal Antibody, Brilliant Ultra Violet™ 395 dye (Cat. No. 363-0036-42) (right). Cells in the lymphocyte gate were used for analysis.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.

For Research Use Only. Not for use in diagnostic procedures.