Brilliant Ultraviolet 737 dye

Brilliant Ultra Violet™ dyes, including Brilliant Ultra Violet™ 737 (BUV737), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors allowing for larger panels.

BUV737 is a tandem dye excited by the 355 nm UV laser and emits at 737 nm far red. This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining. BUV737 may have some cross-laser excitation with the blue and red laser.

When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend that you use Super Bright Complete Staining Buffer (Cat. No. SB-4401-42) or Brilliant Stain Buffer (Cat. No. 00-4409-42) to minimize any non-specific polymer interactions.

BUV737 dye dashboard


Initial brightness

 
 
 
 
 
The UV laser-excitable BUV737 dye is optimized for use both cell surface and intracellular flow applications. Can be used for spectral flow cytometry applications. 

Photostability in buffer

 
 
 
 
 

 355740/35348737 
 
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BUV737 dye products

We offer BUV737 dye conjugated to primary antibodies for use in flow cytometry.

Antibody conjugates

Experimental data using BUV737 dye products

Figure 1. Excitation and emission of Brilliant Ultra Violet™ 737 dye (BUV737).
 

Figure 2. Spectral signature of BUV737 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with clone RM4-5 (mCD4) conjugated to BUV737 were used for analysis.
 

Dot plot of IFN-gamma-BUV737 vs CD4-PE in unstimulated and stimulated cells

Figure 3. C57BL/6 mouse splenocytes were stimulated for 72 hours with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Cat. No. 16-0031-85 and Cat. No. 16-0281-86, respectively). Cells were restimulated for 5 hours with Brefeldin A (Cat. No. 00-4506-51) (left) or restimulated for 5 hours with Cell Stimulation Cocktail (Cat. No. 00-4970-93) and Brefeldin A (right). Cells were then stained intracellularly using the Intracellular Fixation & Permeabilization Buffer Set (Cat. No. 88-8824-00) with CD4 Monoclonal Antibody, PE (Cat. No. 12-0042-82) and 0.5 µg of IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 737 (Cat. No. 367-7311-80). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Cat. No. L34964).

Dot plots of samples stained with IgG1 control vs CD3-FITC and CD4-BUV737 vs CD3-FITC

Figure 4. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Cat. No. 11-0038-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 737 (Cat. No. 367-4714-81) (left) or CD4 Monoclonal Antibody, Brilliant Ultra Violet™ 737 (Cat. No. 367-0047-41) (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50).

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.