Brilliant Ultra Violet™ dyes, including Brilliant Ultra Violet™ 805 (BUV805), are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.

BUV805 is a tandem dye excited by the 355 nm UV laser and emits at 805 nm far red. This dye has a low-to-medium relative brightness and can be used for cell surface and intracellular staining. Nuclear targets may not be expressed at high enough levels to be paired with this dye.

When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend that you use Super Bright Complete Staining Buffer (Cat. No. SB-4401) or Brilliant Stain Buffer (Cat. No. 00-4409-42) to minimize any non-specific polymer interactions.

BUV805 dye dashboard


Initial brightness

 
 
 
 
 
The UV laser-excitable BUV805 dye is optimized for use for both cell surface and intracellular flow applications. Can be used for spectral flow cytometry applications. 

Photostability in buffer

 
 
 
 
 

 355780/60348805 
 
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BUV805 dye products

We offer BUV805 dye conjugated to primary antibodies for use in flow cytometry.

Antibody conjugates

Experimental data using BUV805 dye products

Figure 1. Absorption and fluorescence emission spectra of Brilliant Ultra Violet™ 805 dye (BUV805).
 

Figure 2. Spectral signature of BUV805 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with clone RM4-5 (mCD4) conjugated to BUV805 were used for analysis.
 

Dot plot of IFN-gamma-BUV805 vs CD4-APC in unstimulated and stimulated cells

Figure 3. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors; Cat. No. 00-4975-93) (right). Cells were then stained intracellularly using the Intracellular Fixation & Permeabilization Buffer Set (Cat. No. 88-8824-00) with CD4 Monoclonal Antibody, APC (Cat. No. 17-0047-42) and IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 805 (Cat. No. 368-7311-80). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Cat. No. L34964).

Dot plots of samples stained with IgG1 control vs CD3-FITC and CD4-BUV805 vs CD3-FITC

Figure 4. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Cat. No. 11-0038-42) and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 805 (Cat. No. 368-4714-81) (left) or CD4 Monoclonal Antibody, Brilliant Ultra Violet™ 805 (Cat. No. 368-0047-41) (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50).

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.