graph of emission spectra for eFluor 506 dye

The eFluor 506 organic dye is a violet-laser excitable fluorophore that has an emission peak of 506 nm. With similar spectra characteristics to AmCyan and Horizon V500, conjugates of the eFluor 506 dye require no change to your instrument configuration for use.

Benefits of eFluor 506 include

  • Direct alternative for AmCyan and Horizon V500 fluorophores
  • Similar or better fluorescence intensity than other 500 nm-emitting organic dyes
  • When combined with all five Super Bright dye antibody conjugates, can provide 6 detection channels of the violet laser

eFluor 506 dye dashboard


Initial brightness

 
 
 
 
 
The violet laser–excitable eFluor 506 dye is optimized for use in flow cytometry applications. Detected using typical filters for AmCyan or Horizon V500 dyes, eFluor 506 dye–labeled conjugates are easily added to your multicolor panels.  
   

Photostability in buffer

 
 
 
 
 

  405 525/50 405 506  
 
Laser line

Common filter set

Excitation max

Emission max
 
Flow histogram comparing eFluor 506 dye to Horizon V500 dye

Fluorescence intensity comparison. Mouse splenocytes were stained with CD4 (clone RM4-5) conjugated to eFluor 506 dye (purple line) or to Horizon V500 (blue histogram), using the same concentration of antibody.

Flow histogram comparing eFluor 506 dye before and after fixation
Stability of eFluor 506 dye. Mouse splenocytes stained with Anti-CD4 (clone RM4-5) and either left unfixed (blue line), or were fixed for 30 minutes in Foxp3/Transcription Factor buffer set (purple line) or 30 minutes in Intracellular (IC) Fixation buffer followed by methanol (orange line).

eFluor 506 dye products

We offer eFluor 506 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

Resources

Primary antibodies to match your cytometer's lasers

Cell stain tool

To support your multicolor flow cytometry experiments, our antibody portfolios include a variety of dyes with compatibility to all commonly used flow cytometry lasers.

Fluorescence SpectraViewer

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Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.