Novafluor Blue 510 excitation shown as dotted line and emission shown as solid green histogram
2488506/18496511(in buffer) 5flow cytometry

NovaFluor Blue 510 dye can be used as an alternative to BD Horizon Brilliant Blue (BB515) with a similar excitation spectrum and a cleaner emission spectrum. For example, in flow cytometry panels that include both FITC and BB515, replacing BB515 with NovaFluor Blue 510 dye reduces the complexity score, thus simplifying spectral unmixing of the panel. NovaFluor Blue 510 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. With distinct spectral signatures, NovaFluor Blue 510, NovaFluor Blue 530, and NovaFluor Blue 555 dyes can be used together in a flow cytometry panel. The macromolecule-based NovaFluor Blue 510 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE, and APC tandems observed with macrophages and monocytes.

See all NovaFluor Blue 510 Primary Antibodies

Graph of relative intensity vs wavelength for NovaFluor Blue 510 dye

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 510 dye.

Graph of fluorescence intensity vs channel for NovaFluor Blue 510 dye

Figure 2. Spectral signature of NovaFluor Blue 510 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 510 dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.

Side scatter plot with unstained and CD16 NovaFluor Blue 510-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD16 Monoclonal Antibody, Nova Fluor Blue 510 (right). Data were acquired in the B1 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.

NovaFluor Blue 510 dye background

NovaFluor dyes, including NovaFluor Blue 510 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyesexhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunityto detect additional markers in flow cytometry panels by opening up previously unusable channels.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Additional resources


Flow cytometry protocols handbook

Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.

Request the handbook


Fluorophore and reagent selection guide for flow cytometry

A handy reference poster featuring the broad range of our available dyes and labeling reagents.

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Imaging protocol handbook

Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.

Request the handbook



Fluorophore and reagent selection guide for cell imaging

A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.

 Download the guide

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