|2||488||506/18||496||511||(in buffer) 5||flow cytometry|
NovaFluor Blue 510 dye can be used as an alternative to BD Horizon Brilliant Blue (BB515) with a similar excitation spectrum and a cleaner emission spectrum. For example, in flow cytometry panels that include both FITC and BB515, replacing BB515 with NovaFluor Blue 510 dye reduces the complexity score, thus simplifying spectral unmixing of the panel. NovaFluor Blue 510 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. With distinct spectral signatures, NovaFluor Blue 510, NovaFluor Blue 530, and NovaFluor Blue 555 dyes can be used together in a flow cytometry panel. The macromolecule-based NovaFluor Blue 510 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE, and APC tandems observed with macrophages and monocytes.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 510 dye.
Figure 2. Spectral signature of NovaFluor Blue 510 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 510 dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD16 Monoclonal Antibody, Nova Fluor Blue 510 (right). Data were acquired in the B1 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
NovaFluor dyes, including NovaFluor Blue 510 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyesexhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunityto detect additional markers in flow cytometry panels by opening up previously unusable channels.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.