NovaFluor Blue 530 Dye

NovaFluor dyes, including NovaFluor Blue 530 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

With a similar excitation spectrum and narrower emission spectrum, NovaFluor Blue 530 dye is a good alternative for other dyes with similar emission profiles that are excited by the 488 nm blue laser, such as FITC and Alexa Fluor 488 dye. NovaFluor Blue 530 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. NovaFluor Blue 530 dye can be used in flow cytometry panels that include both NovaFluor Blue 510 and NovaFluor Blue 555 dyes. The macromolecule-based NovaFluor Blue 530 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Blue 530 dye:

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Can serve as a replacement for FITC or Alexa Fluor 488 dye with a cleaner emission spectra
  • Can be used in a flow cytometry panel with NovaFluor Blue 510 and NovaFluor Blue 555 dyes
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Blue 530 Dye dashboard

NovaFluor 530 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services

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Experimental data using NovaFluor Blue 530 dye products
 

Graph of relative intensity vs wavelength for NovaFluor Blue 530 dye

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 530 dye.
 

Graph of fluorescence intensity vs channel for NovaFluor Blue 530 dye

Figure 2. Spectral signature of NovaFluor Blue 530 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 530 dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
 

Side scatter plot with unstained and CD19 NovaFluor Blue 530-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Blue 530 (Cat. No. H004T03B02) (right). Data were acquired in the B2 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.