NovaFluor dyes, including NovaFluor Blue 555 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Blue 555 dye has an excitation and emission profile similar to that of Alexa Fluor 532 dye, but it is 2.5 times brighter. NovaFluor Blue 555 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. NovaFluor 555 dye can be used in flow cytometry panels that include both NovaFluor Blue 510 and NovaFluor Blue 530 dyes. The macromolecule-based NovaFluor Blue 555 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
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Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 555 dye.
Figure 2. Spectral signature of NovaFluor Blue 555 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 555 dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Blue 555 (Cat. No. H004T03B03) (right). Data were acquired in the B3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
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