|(in buffer) 5
NovaFluor Blue 610 dyes are available with two different levels of brightness: NovaFluor Blue 610-30S and NovaFluor Blue 610-70S, allowing for differential matching of the dye brightness to the antigen density or abundance, without panel redesign. NovaFluor Blue 610-30S and NovaFluor Blue 610-70S dyes have separate spectral signatures but are difficult to use in the same panel. NovaFluor Blue 610-30S is dimmer than NovaFluor 610-70S.
NovaFluor Blue 610-30S dye can be used to replace PE/Dazzle 594, BD Horizon PE-CF594, or PE-Texas Red dye using the 488 nm blue laser line for excitation. As compared with these PE tandem dyes, NovaFluor Blue 610-30S dye shows much less cross-excitation and lower spectral spillover with the 561 nm yellow-green laser line, allowing detection of an additional marker labeled with, for example, NovaFluor Yellow 610 dye. The macromolecule-based NovaFluor Blue 610 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 610-30S dye.
Figure 2. Spectral signature of NovaFluor Blue 610-30S dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 610-30S dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Blue 610-30S (right). Data were acquired in the B6 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
NovaFluor dyes, including NovaFluor Blue 610 dyes, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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For Research Use Only. Not for use in diagnostic procedures.