NovaFluor dyes, including NovaFluor Blue 660 dyes, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Blue 660 dyes are available with two different levels of brightness—NovaFluor Blue 660-40S and NovaFluor Blue 660-120S—allowing for differential matching of the dye brightness to the antigen density or abundance without panel redesign. NovaFluor Blue 660-40S and NovaFluor Blue 660-120S dyes have separate spectral signatures, but are difficult to use in the same panel. NovaFluor Blue 660-40S is dimmer than NovaFluor 660-120S.
NovaFluor Blue 660-40S dye can be used to replace PE-tandem dyes, such as PE-Cy5 and PE/Fire™ 640, using the 488 nm blue laser line for excitation. Because PE-tandem dyes are cross-excited by both the blue and yellow-green lasers, the replacement of these dyes with NovaFluor Blue 660-40S dye can allow detection of an additional marker labeled with, for example, NovaFluor Yellow 660 dye. The macromolecule-based NovaFluor Blue 660 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
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Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 660-40S dye.
Figure 2. Spectral signature of NovaFluor Blue 660-40S dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 660-40S dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Blue 660-40S (Cat. No. H004T03B07) (right). Data were acquired in the B7 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
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