NovaFluor Red 685 dye

NovaFluor dyes, including NovaFluor Red 685 dye, are built using Phiton technology and are compatible with both spectral flow cytometry, as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Red 685 dye is ~4 times brighter than APC-Cyanine 5.5 and Alexa Fluor 680 dye. In complex multicolor flow cytometry panels, NovaFluor Red 685 dye can be used to fill the spectral gap between Alexa Fluor 647 and Alexa Fluor 700 dyes. The macromolecule-based NovaFluor Red 685 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Red 685 dye:

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Can be used to fill the spectra gap between Alexa Fluor 647 and Alexa Fluor 700 dyes
  • Can be used in a flow cytometry panel with NovaFluor Red 660 (or APC-Alexa Fluor 647) and Alexa Fluor 700 dyes
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Red 685 dye dashboard


Initial brightness

 
 
 
 
 
  

Photostability in buffer

 
 
 
 
 

NovaFluor Red 685 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Learn more here:

Experimental data using NovaFluor Red 685 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Red 685 dye.

Figure 2. Spectral signature of NovaFluor Red 685. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Red 685 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Side scatter plot with unstained and CD16 NovaFluor Red 685 dye-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD16 Monoclonal Antibody, NovaFluor Red 685 (Cat. No. H006T03R02) (right). Data was acquired in the R3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.