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5 | 637 | 720/30 | 636 | 725 | (in buffer) 4 | flow cytometry |
NovaFluor Red 725 dye is excited by the red laser (637 nm) and is optimized for use for cell surface applications. It can be used for spectral and conventional flow cytometry applications. With its distinct emission spectrum, it fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally distinct dyes may allow for building out larger panels with minimal loss of resolution. NovaFluor Red 725 dye should generally be paired with moderately to lowly expressed antigens to minimize spectral spillover in complex multicolor panels.
The macromolecule-based NovaFluor Red 725 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE, and APC tandems observed with macrophages and monocytes.
Thermo Fisher Scientific offers NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Spectral signature of NovaFluor Red 725 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 725 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Staining of mouse splenocytes with NovaFluor Red 725 conjugated antibody. C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody (OKT3), eFluor 450 only (left) or along with CD45 Monoclonal Antibody (HI30), NovaFluor Red 725 (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation.
Staining of human PBMCs with NovaFluor Red 725 conjugated antibody. Normal human peripheral blood cells (PBMCs) were stained with CD19 Monoclonal Antibody (HIB19), eFluor 450 only (left) or with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 725 (right). Cells in the lymphocyte gate were used for analysis.
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.