NovaFluor Red 725 excitation shown as dotted line and emission shown as solid red histogram
5637720/30636725(in buffer) 4
flow cytometry

NovaFluor Red 725 dye is excited by the red laser (637 nm) and is optimized for use for cell surface applications. It can be used for spectral and conventional flow cytometry applications. With its distinct emission spectrum, it fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally distinct dyes may allow for building out larger panels with minimal loss of resolution. NovaFluor Red 725 dye should generally be paired with moderately to lowly expressed antigens to minimize spectral spillover in complex multicolor panels.

The macromolecule-based NovaFluor Red 725 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE, and APC tandems observed with macrophages and monocytes.

Thermo Fisher Scientific offers NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Red 725 primary antibodies

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Benefits of NovaFluor Red 725 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Should be paired with moderately expressed antigens to minimize spillover
  • Spectrally unique and can be used to replace dim dyes, such as Alexa Fluor 700 in conventional flow cytometry
  • Produces stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Red 725 dye

Spectral signature of NovaFluor Red 725 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 725 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
 

Staining of mouse splenocytes with NovaFluor Red 725 conjugated antibody. C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody (OKT3), eFluor 450 only (left) or along with CD45 Monoclonal Antibody (HI30), NovaFluor Red 725 (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation.
 

Staining of human PBMCs with NovaFluor Red 725 conjugated antibody. Normal human peripheral blood cells (PBMCs) were stained with CD19 Monoclonal Antibody (HIB19), eFluor 450 only (left) or with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 725 (right). Cells in the lymphocyte gate were used for analysis.

NovaFluor dyes

NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

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