NovaFluor Yellow 570 dye

NovaFluor dyes, including NovaFluor Yellow 570 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Yellow 570 dye can be used as an alternative to phycoerythrin (PE) with similar emission spectrum but far less cross-laser excitation by the blue 488 nm laser. NovaFluor Yellow 570 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. The macromolecule-based NovaFluor Yellow 570 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Yellow 570 dye:

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Can serve as a replacement for PE with far less cross-laser excitation
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Yellow 570 dye products


Initial brightness

 
 
 
 
 
  

Photostability in buffer

 
 
 
 
 

NovaFluor Yellow 570 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

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Experimental data using NovaFluor Yellow 570 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 570 dye.

Figure 2. Spectral signature of NovaFluor Yellow 570 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 570 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.

Side Scatter plot with unstained and CD19 NovaFluor Yellow 570-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Yellow 570 (Cat. No. H004T03Y01) (right). Data was acquired in the YG1 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.