NovaFluor dyes, including NovaFluor Yellow 570 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Yellow 570 dye can be used as an alternative to phycoerythrin (PE) with similar emission spectrum but far less cross-laser excitation by the blue 488 nm laser. NovaFluor Yellow 570 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. The macromolecule-based NovaFluor Yellow 570 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 570 dye.
Figure 2. Spectral signature of NovaFluor Yellow 570 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 570 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Yellow 570 (Cat. No. H004T03Y01) (right). Data was acquired in the YG1 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
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