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NovaFluor Yellow 590 Dye
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| 4 | 561 | 590/40 | 563 | 594 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Yellow 590 dye has a unique emission spectrum, which fits in between PE and PE-tandems such as PE-Dazzle 594, potentially allowing the detection of an additional marker in a complex multicolor panel. However, due to spectral spillover by PE and PE-tandems, an optimal panel design would replace PE and PE tandems with NovaFluor Yellow 570 and NovaFluor Yellow 610 dyes to reduce spillover into NovaFluor Yellow 590 dye channel. This dye should generally be paired with medium to highly expressed antigens to minimize spectral spillover in complex multicolor panels.
The macromolecule-based NovaFluor Yellow 590 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Should be paired with medium to highly expressed antigens to minimize spillover
- Can allow the detection of an additional marker between PE and PE-Tandems or NovaFluor Yellow 570 and NovaFluor Yellow 610
- Produces highly stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Yellow 590 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 590 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Staining of human peripheral blood cells with NovaFluor Yellow 590 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD4 Monoclonal Antibody, NovaFluor Yellow 590 (right). Data was acquired in the YG2 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
NovaFluor dyes
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.