NovaFluor dyes, including NovaFluor Yellow 610 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Yellow 610 dye can be used to replace PE/Dazzle™ 594, BD Horizon™ PE-CF594, or PE-Texas Red dye using the 561 nm yellow laser line for excitation. As compared with these PE tandem dyes, NovaFluor Yellow 610 dye shows much less cross-excitation and lower spectral spillover with the 488 nm blue laser line, allowing detection of an additional marker labeled with, for example, one of the NovaFluor Blue 610 dyes. The macromolecule-based NovaFluor Yellow 610 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 610 dye.
Figure 2. Spectral signature of NovaFluor Yellow 610 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 610 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD16 Monoclonal Antibody, NovaFluor Yellow 610 (Cat. No. H006T03Y03) (right). Data was acquired in the YG3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
PE/Dazzle™ is a trademark of BioLegend, BD Horizon™ is a trademark of BD Bioscience.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
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For Research Use Only. Not for use in diagnostic procedures.