NovaFluor Yellow 660 dye

NovaFluor Yellow 660 dye has been optimized for flow cytometers with spectral capabilities. Phiton technology provides highly stable fluorescence when stored at 4°C. NovaFluor Yellow 660 can be used to replace PE-tandems such as PE-Cy5 or PE-FireTM 640, with much less cross-excitation and fluorescent spillover. Replacement of PE-tandems with NovaFluor Yellow 660, which is primarily excited by the yellow-green laser, opens a detector, allowing for an additional marker to be detected off of the blue laser line using NovaFluor Blue 660.

Benefits of NovaFluor Yellow 660 dye include:

  • Can be paired with medium to lowly expressed antigens
  • Replacement for PE-Cy5 with reduced cross-laser excitation and fluorescent spillover
  • PE-Cy5 can be replaced by NovaFluor Blue 660 and NovaFluor Yellow 660 for larger conventional flow panels
  • Highly stable and compatible with all tested buffers

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Yellow 660 dye dashboard


Initial brightness

 
 
 
 
 
  

Photostability in buffer

 
 
 
 
 
  

NovaFluor Yellow 660 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

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Experimental data using NovaFluor Yellow 660 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 660 dye.

Figure 2. Spectral signature of NovaFluor Yellow 660 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 660 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.

Side Scatter plot with unstained and CD3 NovaFluor Yellow 660-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD3 Monoclonal Antibody, NovaFluor Yellow 660 (Cat. No. H002T03Y04) (right). Data was acquired in the YG4 channel on a 5-laser Cytek Aurora and singlet cells were used for analysis.
 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.