NovaFluor Yellow 660 dye has been optimized for flow cytometers with spectral capabilities. Phiton technology provides highly stable fluorescence when stored at 4°C. NovaFluor Yellow 660 can be used to replace PE-tandems such as PE-Cy5 or PE-FireTM 640, with much less cross-excitation and ﬂuorescent spillover. Replacement of PE-tandems with NovaFluor Yellow 660, which is primarily excited by the yellow-green laser, opens a detector, allowing for an additional marker to be detected off of the blue laser line using NovaFluor Blue 660.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 2. Spectral signature of NovaFluor Yellow 660 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 660 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD3 Monoclonal Antibody, NovaFluor Yellow 660 (Cat. No. H002T03Y04) (right). Data was acquired in the YG4 channel on a 5-laser Cytek Aurora and singlet cells were used for analysis.
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For Research Use Only. Not for use in diagnostic procedures.