NovaFluor dyes, including NovaFluor Yellow 690 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Yellow 690 dye has an emission spectrum that fits between the emission peaks of PE-Cyanine 5 and PE-Cyanine 7, potentially allowing detection of an additional marker in a complex multicolor panel. NovaFluor Yellow 690 and NovaFluor Yellow 700 dyes cannot be used together with conventional flow cytometry, but their distinct spectral signatures allow them to be successfully unmixed using a spectral flow cytometer. The macromolecule-based NovaFluor Yellow 690 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Yellow 690 (Cat. No. H004T03Y05) (right). Data was acquired in the YG6 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.