NovaFluor Yellow 690 dye

NovaFluor dyes, including NovaFluor Yellow 690 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Yellow 690 dye has an emission spectrum that fits between the emission peaks of PE-Cyanine 5 and PE-Cyanine 7, potentially allowing detection of an additional marker in a complex multicolor panel. NovaFluor Yellow 690 and NovaFluor Yellow 700 dyes cannot be used together with conventional flow cytometry, but their distinct spectral signatures allow them to be successfully unmixed using a spectral flow cytometer. The macromolecule-based NovaFluor Yellow 690 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Yellow 690 dye:

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Can allow the detection of an additional marker between PE-Cy5 and PE-Cy7 emissions
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Yellow 690 dye dashboard


Initial brightness

 
 
 
 
 
  

Photostability in buffer

 
 
 
 
 


NovaFluor Yellow 690 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

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Experimental data using NovaFluor Yellow 690 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 690 dye.
Figure 2. Spectral signature of NovaFluor Yellow 690 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 690 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Side Scatter plot with unstained and CD19 NovaFluor Yellow 690 stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Yellow 690 (Cat. No. H004T03Y05) (right). Data was acquired in the YG6 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.