NovaFluor dyes, including NovaFluor Yellow 730 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral interference for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Yellow 730 dye can be used as an alternative to PE-Cyanine 5.5, with far less cross-laser excitation and spectral spillover. NovaFluor Yellow 690, NovaFluor Yellow 700 and NovaFluor 730 dyes cannot be used together with conventional flow cytometry, but their distinct spectral signatures allow them to be successfully unmixed using a spectral flow cytometer. The macromolecule-based NovaFluor Yellow 700 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 730 dye.
Figure 2. Spectral signature of NovaFluor Yellow 730 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 730 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD4 Monoclonal Antibody, NovaFluor Yellow 730 (Cat. No. H001T03Y07) (right). Data was acquired in the YG7 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
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For Research Use Only. Not for use in diagnostic procedures.