NovaFluor dyes, including NovaFluor Yellow 755 dye, are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Yellow 755 dye has a unique emission spectrum when excited by the 561nm laser, which fits in a spectral space that is currently only accessible using cross-laser excited tandem dyes, such as PE-Cyanine7. Replacing highly cross-laser excited dyes may reduce spread, allowing for better resolution of low expressed markers. NovaFluor Yellow 755 dye should generally be paired with moderately expressed antigens to minimize spectral spillover in complex multicolor panels. The macromolecule-based NovaFluor Yellow 755 dye produces stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 755 dye.
Figure 2. Spectral signature of NovaFluor Yellow 755 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 755 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system
Figure 3. C57BL/6 mouse splenocytes were stained with CD3e monoclonal antibody, eFluor 450 dye (Cat. No. 48-0037-42) only (left) or along with CD45R monoclonal antibody, NovaFluor Yellow 755 dye (Cat. No. H029T03Y08) (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Cat. No. L34962).
Figure 4. Normal human peripheral blood cells were stained with CD19 monoclonal antibody, eFluor 450 dye (Cat. No. 48-0199-42) only (left) or with CD4 monoclonal antibody, NovaFluor Yellow 755 dye (Cat. No. H001T03Y08) (right). Cells in the lymphocyte gate were used for analysis.
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