Oregon Green 488 spectra excitation shown as dotted line and emission shown as solid green histogram
4488FITC498526(in buffer) 3
(in antifade) 4
microscopy, flow cytometry, fluorescence microplate reader

Invitrogen Oregon Green 488 is a bright, green-fluorescent dye with excitation well suited for the 488 nm laser line. It has a high extinction coefficient and fluorescence quantum yield. While it is pH insensitive in the physiological range, with a pKa of 4.6, it can be used to monitor pH change in acidic organelles or pathways. Oregon Green 488 dye can be used to label a number of protein conjugates and lipid probes and it also forms the basis for a series of ion indicators used for physiological analysis, including calcium. High-performance alternativesOregon Green 488 dye include Alexa Fluor 488 Dye and pHrodo Green Indicators.

We offer Oregon Green 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own protein, conjugates or probes.

Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)  Search Oregon Green 488 primary antibodies

A confocal laser-scanning microscope image of microtubules visualized with Oregon Green 488 paclitaxel in live HeLa cells

Microtubules visualized with Oregon Green 488 paclitaxel. Microtubules were assembled, stabilized and visualized with using green-fluorescent Paclitaxel, Oregon Green 488 Conjugate. Viable HeLa cells were incubated with the conjugate for one hour, followed by several washes with phosphate-buffered saline containing 2% BSA. The image was acquired using a confocal laser-scanning microscope and a filter set appropriate for fluorescein.

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For Research Use Only. Not for use in diagnostic procedures.