graph of emission spectra for PerCP-eFluor 710 dye

The PerCP-eFluor 710 tandem dye was developed as an optimal choice for the FL3 channel on a blue laser line. It is consistently two to three times brighter than PerCP-Cyanine5.5 when evaluated in clone-to-clone comparison tests on identical samples. Although PerCP-eFluor 710 is slightly red-shifted in its emission compared to PerCP-Cyanine5.5 (710 nm vs. 685 nm), it uses the same filter sets.

Benefits of PerCP-eFluor 710 include

  • Greater mean fluorescence intensity (MFI) as compared to PerCP-Cyanine5.5
  • Minimizes the need for compensation out of the PE detector
  • Stable performance when treated with formaldehyde fixation or exposed to ambient light

PerCP-eFluor 710 dye dashboard


Initial brightness

 
 
 
 
 
The blue laser–excitable PerCP-eFluor 710 tandem dye is designed for use in flow cytometry applications. No change to your instrumentation is needed if you already use PE-Cy5.5 or PerCP-Cy5.5, allowing you to easily add PerCP-eFluor 710 to your multicolor panels.  
   

Photostability in buffer

 
 
 
 
 

  488 710/50 633 710  
 
Laser line

Common filter set

Excitation max

Emission max
 
Flow histogram comparing PerCP-eFluor 710 dye to PerCP-Cyanine5.5 dye

Staining performance compared to PerCP-Cyanine5.5. Direct comparison of mouse splenocytes stained with anti-mouse CD4 (clone RM4-5) conjugated to either PerCP-eFluor 710 dye (red line) or PerCP-Cyanine5.5 (pink line).

Flow histogram comparing PerCP-eFluor 710 dye before and after aldehyde fixation or extended ambient light exposure

Effects of light exposure or fixation on PerCP-eFluor 710 dye. Human PBMCs were stained with Anti-CD4 PerCP-eFluor 710 dye (clone SK3) and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde overnight at 4°C (green line) and analyzed again.

PerCP-eFluor 710 dye products

We offer PerCP-eFluor 710 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

See all PerCP-eFluor 710 Primary Antibodies
See all PerCP-eFluor 710 Secondary Antibodies

Resources

Primary antibodies to match your cytometer's lasers

Cell stain tool

To support your multicolor flow cytometry experiments, our antibody portfolios include a variety of dyes with compatibility to all commonly used flow cytometry lasers.

Fluorescence SpectraViewer

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Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.