The PerCP-eFluor 710 tandem dye was developed as an optimal choice for the FL3 channel on a blue laser line. It is consistently two to three times brighter than PerCP-Cyanine5.5 when evaluated in clone-to-clone comparison tests on identical samples. Although PerCP-eFluor 710 is slightly red-shifted in its emission compared to PerCP-Cyanine5.5 (710 nm vs. 685 nm), it uses the same filter sets.
Benefits of PerCP-eFluor 710 include
- Greater mean fluorescence intensity (MFI) as compared to PerCP-Cyanine5.5
- Minimizes the need for compensation out of the PE detector
- Stable performance when treated with formaldehyde fixation or exposed to ambient light
PerCP-eFluor 710 dye dashboard
|The blue laser–excitable PerCP-eFluor 710 tandem dye is designed for use in flow cytometry applications. No change to your instrumentation is needed if you already use PE-Cy5.5 or PerCP-Cy5.5, allowing you to easily add PerCP-eFluor 710 to your multicolor panels.|
Photostability in buffer
Staining performance compared to PerCP-Cyanine5.5. Direct comparison of mouse splenocytes stained with anti-mouse CD4 (clone RM4-5) conjugated to either PerCP-eFluor 710 dye (red line) or PerCP-Cyanine5.5 (pink line).
Effects of light exposure or fixation on PerCP-eFluor 710 dye. Human PBMCs were stained with Anti-CD4 PerCP-eFluor 710 dye (clone SK3) and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde overnight at 4°C (green line) and analyzed again.
PerCP-eFluor 710 dye products
We offer PerCP-eFluor 710 dye conjugated to primary antibodies for use in flow cytometry.
Learn more here:
Primary antibodies to match your cytometer's lasers
To support your multicolor flow cytometry experiments, our antibody portfolios include a variety of dyes with compatibility to all commonly used flow cytometry lasers.
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.