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PerCP-eFluor 710 Dye
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| 4 | 488 | 710/50 | 482 | 708 | (in buffer) 1 | flow cytometry |
Invitrogen PerCP-eFluor 710 is a blue laser–excitable tandem dye designed for use in flow cytometry applications. It was developed as an optimal choice for the FL3 channel on a blue laser line. It is consistently two to three times brighter than PerCP-Cyanine5.5 when evaluated in clone-to-clone comparison tests on identical samples. Although PerCP-eFluor 710 is slightly red-shifted in its emission compared to PerCP-Cyanine5.5 (710 nm vs. 685 nm), it uses the same filter sets. No change to your instrumentation is needed if you already use PE-Cy5.5 or PerCP-Cy5.5, allowing you to easily add PerCP-eFluor 710 to your multicolor panels.
We offer PerCP-eFluor 710 dye conjugated to a variety of antibodies, proteins, and amplification substrates optimized for cellular labeling and detection.
Search PerCP-eFluor 710 primary antibodies Search PerCP-eFluor 710 secondary antibodies
Additional products
- Greater mean fluorescence intensity (MFI) as compared to PerCP-Cyanine5.5
- Minimizes the need for compensation out of the PE detector
- Stable performance when treated with formaldehyde fixation or exposed to ambient light
Comparison of staining performance of PerCP-eFluor 710 to PerCP-Cyanine5.5. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), PerCP-eFluor 710 (red line) or CD4 Monoclonal Antibody (RM4-5), PerCP-Cyanine5.5 (pink line).
Stability of PerCP-eFluor 710 dye. Human peripheral blood cells (PBMCs) were stained with CD4 Monoclonal Antibody (SK3 (SK-3)), PerCP-eFluor 710 and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde overnight at 4°C (green line) and analyzed again.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
Stylesheet for Classic Wide Template adjustments