graph of emission spectra for PerCP-eFluor 710 dye

The PerCP-eFluor 710 tandem dye was developed as an optimal choice for the FL3 channel on a blue laser line. It is consistently two to three times brighter than PerCP-Cyanine5.5 when evaluated in clone-to-clone comparison tests on identical samples. Although PerCP-eFluor 710 is slightly red-shifted in its emission compared to PerCP-Cyanine5.5 (710 nm vs. 685 nm), it uses the same filter sets.

Benefits of PerCP-eFluor 710 include

  • Greater mean fluorescence intensity (MFI) as compared to PerCP-Cyanine5.5
  • Minimizes the need for compensation out of the PE detector
  • Stable performance when treated with formaldehyde fixation or exposed to ambient light

PerCP-eFluor 710 dye dashboard


Initial brightness

 
 
 
 
 
The blue laser–excitable PerCP-eFluor 710 tandem dye is designed for use in flow cytometry applications. No change to your instrumentation is needed if you already use PE-Cy5.5 or PerCP-Cy5.5, allowing you to easily add PerCP-eFluor 710 to your multicolor panels.  
   

Photostability in buffer

 
 
 
 
 

  488 710/50 633 710  
 
Laser line

Common filter set

Excitation max

Emission max
 
Flow histogram comparing PerCP-eFluor 710 dye to PerCP-Cyanine5.5 dye

Staining performance compared to PerCP-Cyanine5.5. Direct comparison of mouse splenocytes stained with anti-mouse CD4 (clone RM4-5) conjugated to either PerCP-eFluor 710 dye (red line) or PerCP-Cyanine5.5 (pink line).

Flow histogram comparing PerCP-eFluor 710 dye before and after aldehyde fixation or extended ambient light exposure

Effects of light exposure or fixation on PerCP-eFluor 710 dye. Human PBMCs were stained with Anti-CD4 PerCP-eFluor 710 dye (clone SK3) and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde overnight at 4°C (green line) and analyzed again.

PerCP-eFluor 710 dye products

We offer PerCP-eFluor 710 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

See all PerCP-eFluor 710 Primary Antibodies
See all PerCP-eFluor 710 Secondary Antibodies

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.