SYTO 9 stain is an excellent green-fluorescent nuclear and chromosome counterstain that is permeant to both prokaryotic and eukaryotic cell membranes. SYTO 9 stain has a high affinity for DNA and exhibits enhanced fluorescence upon binding with an excitation maximum at 483 nm and fluorescence emission maximum at 503 nm.
SYTO 9 stain is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead Gram-positive and Gram-negative bacteria.
|Live-cell SYTO dyes are permeant to both eukaryotic and prokaryotic membranes, and exhibit enhanced fluorescence upon binding to both DNA and RNA.|
Photostability in buffer
Photostability in antifade
Common filter set
The SYTO green-fluorescent nucleic acid stains have proven valuable in a broad range of research applications. SYTO 9 stain is particularly used for staining live and dead Gram-positive and Gram-negative bacteria.
Live/dead analysis of Streptomyces antibioticus ETH7451 cultures.
The mycelium was stained with SYTO 9 dye and propidium iodide (components of the LIVE/DEAD BacLight Bacterial Viability Kit, Cat. Nos. L7007, L7012, L13152). Live portions fluoresce green, and membrane-compromised portions fluoresce red. Image contributed by Jesus Sanchez and Marisol Fernandez, Microbiology Area, University of Oviedo, Spain, and reproduced with the permission of Microbiology 148, 405 (2002)
Unlike our range of nuclear counterstains, SYTO dyes do not act exclusively as nuclear stains in live cells and should not be equated with DNA-selective compounds (such as DAPI) which stain nuclei in live animal cells. Eukaryotic cells incubated with SYTO dyes generally show cytoplasmic or mitochondrial staining, as well as nuclear staining.
Nuclear counterstains provide highly selective nuclear staining with little or no cytoplasmic labeling and a choice of colors for multiplexing with other labels. Additionally, some of the stains can also be used in cell cycle analyses using high-content imaging and flow cytometry.
A fixed, permeabilized, and labeled muntjac skin fibroblast. Mitochondria were labeled with mouse anti–OxPhos Complex V inhibitor protein antibody and visualized using orange-fluorescent Alexa Fluor 555 goat anti–mouse IgGantibody. F-actin was labeled with green-fluorescent Alexa Fluor 488 phalloidin, and the nucleus was stained with TO-PRO-3 iodide (pseudocolored magenta).
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