SYTOX® Green stain

SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells but penetrates the compromised membranes characteristic of dead cells, making it a useful indicator of dead cells within a population.

SYTOX Green stain binds nucleic acids with minimal base selectivity. It exhibits a >500-fold fluorescence enhancement upon binding nucleic acids, with an emission peak of 523 nm when excited by a 488 nm argon-ion laser or any other 450 to 490 nm source.

The exceptional brightness of the signal produced in dead cells makes SYTOX Green stain particularly useful for determining viability of both mammalian and bacterial cells.

SYTOX® Green dashboard


Initial brightness

 
 
 
 
 
SYTOX Green stain is useful as a dead-cell stain because of its cell impermeability and >500-fold increase in fluorescence upon nucleic acid binding.  
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

SYTOX Green stain products

We offer a range of SYTOX® Green stain products for nucleic acid staining and dead cell detection in both flow cytometry and imaging applications. SYTOX® Green stain is often included in multiplexed assays for apoptosis or cell viability.

SYTOX® Green stain

Multicolor fluorescence image of mouse intestine cross section. Nuclei were stained with SYTOX Green nucleic acid stain.

Alternatives to SYTOX Green stain

We also offer a range of nucleic acid stains in simple ReadyProbes format and as components in multiplexed viability assays.

  LIVE/DEAD® Cell Imaging Kit
Hep G2 Cells Stained Using the LIVE/DEAD Cell Imaging Kit, showing green-fluorescent live cells and red-fluorescent dead cells.

Resources

Molecular Probes Handbook

The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

Go to the Handbook online
Order your copy of the Handbook

Antifade reagents

Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

Learn more about SlowFade Antifade reagents
Learn more about ProLong Antifade reagents

Cell stain tool

Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

Go to the cell stain tool

Fluorescence SpectraViewer

Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer