SYTOX® Green stain
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells but penetrates the compromised membranes characteristic of dead cells, making it a useful indicator of dead cells within a population.
SYTOX® Green stain binds nucleic acids with minimal base selectivity. It exhibits a >500-fold fluorescence enhancement upon binding nucleic acids, with an emission peak of 523 nm when excited by a 488 nm argon-ion laser or any other 450 to 490 nm source.
The exceptional brightness of the signal produced in dead cells makes SYTOX® Green stain particularly useful for determining viability of both mammalian and bacterial cells.
SYTOX® Green dashboard
SYTOX® Green stain products
We offer a range of SYTOX® Green stain products for nucleic acid staining and dead cell detection in both flow cytometry and imaging applications. SYTOX® Green stain is often included in multiplexed assays for apoptosis or cell viability.
Multicolor fluorescence image of mouse intestine cross section. Nuclei were stained with SYTOX® Green nucleic acid stain.
Alternatives to SYTOX® Green stain
We also offer a range of nucleic acid stains in simple ReadyProbes™ format and as components in multiplexed viability assays.
Hep G2 Cells Stained Using the LIVE/DEAD® Cell Imaging Kit, showing green-fluorescent live cells and red-fluorescent dead cells.