SYTOX Green nucleic acid stain

SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells but penetrates the compromised membranes characteristic of dead cells, making it a useful indicator of dead cells within a population.

SYTOX Green stain binds nucleic acids with minimal base selectivity. It exhibits a >500-fold fluorescence enhancement upon binding nucleic acids, with an emission peak of 523 nm when excited by a 488 nm argon-ion laser or any other 450 to 490 nm source.

The exceptional brightness of the signal produced in dead cells makes SYTOX Green stain particularly useful for determining viability of both mammalian and bacterial cells.

SYTOX Green dashboard


Initial brightness
 
 
 
 
 
SYTOX Green stain is useful as a dead-cell stain because of its cell impermeability and >500-fold increase in fluorescence upon nucleic acid binding. 
  

Photostability in buffer
 
 
 
 
 
  

Photostability in antifade
 
 
 
 
 
 488FITC504523 
 
Laser line
Common filter set
Excitation max
Emission max		 

SYTOX Green stain products

We offer a range of SYTOX Green stain products for nucleic acid staining and dead cell detection in both flow cytometry and imaging applications. SYTOX Green stain is often included in multiplexed assays for apoptosis or cell viability.

SYTOX® Green stain

Multicolor fluorescence image of mouse intestine cross section. Nuclei were stained with SYTOX Green nucleic acid stain.

Alternatives to SYTOX Green stain

We also offer a range of nucleic acid stains in simple ReadyProbes format and as components in multiplexed viability assays.

 LIVE/DEAD® Cell Imaging Kit
Hep G2 Cells Stained Using the LIVE/DEAD Cell Imaging Kit, showing green-fluorescent live cells and red-fluorescent dead cells.