Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells but penetrates the compromised membranes characteristic of dead cells, making it a useful indicator of dead cells within a population.
SYTOX Green stain binds nucleic acids with minimal base selectivity. It exhibits a >500-fold fluorescence enhancement upon binding nucleic acids, with an emission peak of 523 nm when excited by a 488 nm argon-ion laser or any other 450 to 490 nm source.
The exceptional brightness of the signal produced in dead cells makes SYTOX Green stain particularly useful for determining viability of both mammalian and bacterial cells.
Initial brightness | SYTOX Green stain is useful as a dead-cell stain because of its cell impermeability and >500-fold increase in fluorescence upon nucleic acid binding. | ||
Photostability in buffer | |||
Photostability in antifade |
488 | FITC | 504 | 523 | ||
Laser line | Common filter set | Excitation max | Emission max |
We offer a range of SYTOX Green stain products for nucleic acid staining and dead cell detection in both flow cytometry and imaging applications. SYTOX Green stain is often included in multiplexed assays for apoptosis or cell viability.
Multicolor fluorescence image of mouse intestine cross section. Nuclei were stained with SYTOX Green nucleic acid stain.
We also offer a range of nucleic acid stains in simple ReadyProbes format and as components in multiplexed viability assays. | Hep G2 Cells Stained Using the LIVE/DEAD Cell Imaging Kit, showing green-fluorescent live cells and red-fluorescent dead cells. |