Tetramethylrhodamine (TRITC)

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates, as evidenced by over 19,000 citations in the literature. We offer rhodamine reactive dyes as well as other protein and antibody conjugates or probes.

For brighter conjugates and higher photostability, we offer Alexa Fluor 555 as a reactive dye or conjugated to a variety of antibodies, peptides, proteins, tracers, or amplification substrates; all optimized for cellular labeling and detection.

Tetramethylrhodamine (TRITC) dashboard


Initial brightness

 
 
 
 
 
Tetramethylrhodamine is a traditional orange-fluorescent dye which has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.  
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

  488/532 TRITC 557 576  
 
Laser line

Common filter set

Excitation max

Emission max
 

Tetramethylrhodamine (TRITC) products

We offer a broad range of tetramethylrhodamine products including secondary antibodies, bioconjugates, and more.

Tetramethylrhodamine (TRITC)

Zebrafish retina labeled with ELF 97 Immunohistochemistry Kit, Hoechst 33342, and tetramethylrhodamine wheat germ agglutinin.

Tetramethylrhodamine (TRITC) reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

High-performance alternatives to tetramethylrhodamine (TRITC)

Reactive dyes and conjugates

The Invitrogen Alexa Fluor 555 dye—with spectral properties and quantum yield nearly identical to those of tetramethylrhodamine (TRITC) —produces brighter, more photostable conjugates. These conjugates are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.

We offer Alexa Fluor 555 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Learn more about Alexa Fluor 555 dye

Phalloidin conjugates

Fluorescent conjugates of phalloidin using the Invitrogen Alexa Fluor dyes are designed for bright, specific F-actin staining for most applications across the full spectral range. The Invitrogen Alexa Fluor phalloidin conjugates provide researchers with fluorescent probes that are designed to be superior in brightness and photostability to all other spectrally similar conjugates tested.

Learn more about Alexa Fluor phalloidin conjugates

 

Resources

Molecular Probes Handbook

The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

Go to the Handbook online
Order your copy of the Handbook

Antifade reagents

Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

See the performance of this dye in SlowFade Antifade reagents
See the performance of this dye in ProLong Antifade reagents

Cell stain tool

Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

Go to the cell stain tool

Fluorescence SpectraViewer

Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer