Tetramethylrhodamine (TRITC)

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates, as evidenced by over 19,000 citations in the literature. We offer rhodamine reactive dyes as well as other protein and antibody conjugates or probes.

For brighter conjugates and higher photostability, we offer Alexa Fluor® 555 as a reactive dye or conjugated to a variety of antibodies, peptides, proteins, tracers, or amplification substrates; all optimized for cellular labeling and detection.

Tetramethylrhodamine (TRITC) dashboard


Initial brightness

 
 
 
 
 

 


Tetramethylrhodamine is a traditional orange-fluorescent dye which has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.

 
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

  488/532 TRITC 557 576  
 
Laser line

Common filter set

Excitation max

Emission max
 

Tetramethylrhodamine (TRITC) products

We offer a broad range of tetramethylrhodamine products including secondary antibodies, bioconjugates, and more.

Tetramethylrhodamine (TRITC)

Zebrafish retina labeled with ELF® 97 Immunohistochemistry Kit, Hoechst 33342, and tetramethylrhodamine wheat germ agglutinin.

Tetramethylrhodamine (TRITC) reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine Thiol

High-performance alternatives to tetramethylrhodamine (TRITC)

Alexa Fluor® 555

Reactive dyes and conjugates

The Alexa Fluor® 555 dye—with spectral properties and quantum yield nearly identical to those of tetramethylrhodamine (TRITC) —produces brighter, more photostable conjugates. These conjugates are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.

We offer Alexa Fluor® 555 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Phalloidin conjugates

Fluorescent conjugates of phalloidin using the Molecular Probes® Alexa Fluor® dyes are designed for bright, specific F-actin staining for most applications across the full spectral range. The Alexa Fluor® phalloidin conjugates provide researchers with fluorescent probes that are designed to be superior in brightness and photostability to all other spectrally similar conjugates tested.

 

Resources

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with tetramethylrhodamine (TRITC) and a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.

     

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.