APEX® Antibody Labeling Kits utilize a unique solid-phase labeling method that enables an efficient, convenient and reliable method for covalently attaching fluorescent labels to very small amounts of IgG antibody in the presence of any contaminants including stabilizing proteins and amine-containing buffers.
- Reliability—Produces conjugates with stable, covalent attachment of superior fluorescent labels
- Convenience—Requires only 10–20 µg of IgG antibody per labeling reaction, regardless of any purity limitations
- Complete—Includes everything required to perform 5 separate labelings
How APEX® Antibody Labeling Kits work
The APEX® Antibody Labeling Kits use a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX® antibody labeling tip (Figure 1). Any contaminants, including stabilizing proteins or amine-containing buffers, are simply eluted through the tip. After applying the amine-reactive label, a fluorescent IgG conjugate is ready for use in any imaging or flow cytometry application in as little as 2.5 hours with very little hands-on time. The typical yield of labeled antibody using this method ranges from 40–80%.
The APEX® Antibody Labeling Kits includes all reagents required to perform 5 separate labeling reactions of 10–20 μg of IgG antibody with one of Molecular Probes® superior fluorophores or biotin-XX. The biotin-XX provides a 14-atom spacer between the antibody and the biotin moiety.
Figure 1. APEX® antibody labeling tip.
APEX® Antibody Labeling Kits selection guide
|Fluorophore or biotin label||Ex/Em (nm)||Application||Cat. No.|
|Alexa Fluor® 488||496/519||Fluorescent label for use in imaging or flow cytometry; hapten for signal amplification with anti–Alexa Fluor® 488 antibodies.||A10468|
|Alexa Fluor® 555||555/565||Fluorescent label for use in imaging||A10470|
|Alexa Fluor® 568||578/603||A10494|
|Alexa Fluor® 594||590/617||A10474|
|Alexa Fluor® 647||650/665||Fluorescent label for use in imaging or flow cytometry||A10475|
|Oregon Green® 488||496/524||Fluorescent label for use in imaging or flow cytometry; hapten for signal amplification with anti-fluorescein/Oregon Green® antibodies||A10476|
|Pacific Blue™ dye||416/451||Fluorescent label for use in imaging or flow cytometry||A10478|
|Biotin-XX||NA||Streptavidin, avidin, and NeutrAvidin® binding partner for use in imaging or flow cytometry||A10495|
|Ex/Em = Fluorescence excitation and emission maxima, in nm.|
Need to label other antibody amounts?
|IgG amount||Attachment of label||Products|
|<1–20 µg||Site-specific||Zenon® Antibody Labeling Kits|
|<100 µg||Site-specific||SiteClick™ Antibody Labeling Kits|
|10–20 µg||Conventional covalent||APEX® Antibody Labeling Kits|
|20–100 µg||Conventional covalent||Microscale Protein Labeling Kits|
|100 µg||Conventional covalent||Antibody Labeling Kits|
|1 mg||Conventional covalent||Protein Labeling Kits|
|0.5–3 mg||Conventional covalent||SAIVI™ Rapid Antibody Labeling Kits|
For Research Use Only. Not for use in diagnostic procedures.