Molecular Probes® Protein Labeling Kits provide a nearly effortless way to label 1 mg of IgG with a fluorescent dye (including Alexa Fluor® dyes) or biotin.
- Simple—ready to use in 2 hours, requiring approximately 30 minutes actual hands-on time
- Reliable—protein conjugates with stable attachment of fluorescent labels for subsequent use in fluorescence-based imaging or flow cytometry
- Convenient—easiest method to label larger amounts of IgG antibody
The Protein Labeling Kits utilize an amine-reactive fluorophore or hapten to covalently attach the label to the IgG antibody. Once formed, the covalent bond between the label and the protein is extremely stable and you are using the same chemistry we use to prepare our own primary and secondary conjugates.
Simply add ~1 mg of purified IgG (in ~500 μL and free of amine-containing buffers such as Tris) to one of the vials containing a premeasured quantity of amine-reactive dye and a magnetic stir bar. Purification is accomplished on a gravity-feed size exclusion column supplied with the kit (Figure 1). The entire labeling and purification procedure can be completed in as little as 2 hours and everything needed to perform three conjugations is provided.
Figure 1. Molecular Probes® Protein Labeling Kits are the simplest way
to label 1 mg of IgG antibodies.